Simultaneous Determination of Theobromine, Paraxanthine, Theophylline, and Caffeine in Urine by Reversed-Phase High-Performance Liquid Chromatography with Diode Array UV Detection
Küçük Resim Yok
Tarih
2010
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Taylor & Francis Inc
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
A reversed-phase high performance liquid chromatographic method was improved for the simultaneous determination of theobromine, paraxanthine, theophylline, and caffeine in urine. The method includes a liquid-liquid extraction at alkaline pH with ethylacetate. The 7-(2,3-dihidroxypropyl) theophylline was used as an internal standard (ISTD). The separation was achieved on a C18 column using 14:86 methanol:buffer (25mM KH2PO4 adjusted to pH 4 with ortho-phosphoric acid) solution as mobile phase under isocratic conditions at a flow rate 1mLmin-1. An ultraviolet absorption at 274nm was monitored. In these conditions, the LOD was 0.03gmL-1 for theobromine, 0.02gmL-1 for paraxanthine, 0.04gmL-1 for theophylline, and 0.08gmL-1 for caffeine. The method has been applied to urine samples.
Açıklama
Anahtar Kelimeler
Caffeine, Human urine, Paraxanthine, RP-HPLC, DAD, Theobromine, Theophylline
Kaynak
Analytical Letters
WoS Q Değeri
Q4
Scopus Q Değeri
Q3
Cilt
43
Sayı
16
