Simultaneous Determination of Theobromine, Paraxanthine, Theophylline, and Caffeine in Urine by Reversed-Phase High-Performance Liquid Chromatography with Diode Array UV Detection

Basit Öğe Kaydı
dc.authoridBuyuktuncel, Ebru/0000-0001-7269-6594
dc.authorwosidBuyuktuncel, Saliha Ebru/ABH-9343-2020
dc.contributor.authorBuyuktuncel, Ebru
dc.date.accessioned2024-08-04T20:32:40Z
dc.date.available2024-08-04T20:32:40Z
dc.date.issued2010
dc.departmentİnönü Üniversitesien_US
dc.description.abstractA reversed-phase high performance liquid chromatographic method was improved for the simultaneous determination of theobromine, paraxanthine, theophylline, and caffeine in urine. The method includes a liquid-liquid extraction at alkaline pH with ethylacetate. The 7-(2,3-dihidroxypropyl) theophylline was used as an internal standard (ISTD). The separation was achieved on a C18 column using 14:86 methanol:buffer (25mM KH2PO4 adjusted to pH 4 with ortho-phosphoric acid) solution as mobile phase under isocratic conditions at a flow rate 1mLmin-1. An ultraviolet absorption at 274nm was monitored. In these conditions, the LOD was 0.03gmL-1 for theobromine, 0.02gmL-1 for paraxanthine, 0.04gmL-1 for theophylline, and 0.08gmL-1 for caffeine. The method has been applied to urine samples.en_US
dc.identifier.doi10.1080/00032711003731357
dc.identifier.endpage2524en_US
dc.identifier.issn0003-2719
dc.identifier.issn1532-236X
dc.identifier.issue16en_US
dc.identifier.scopus2-s2.0-78649723318en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage2518en_US
dc.identifier.urihttps://doi.org/10.1080/00032711003731357
dc.identifier.urihttps://hdl.handle.net/11616/95200
dc.identifier.volume43en_US
dc.identifier.wosWOS:000284890400006en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Incen_US
dc.relation.ispartofAnalytical Lettersen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCaffeineen_US
dc.subjectHuman urineen_US
dc.subjectParaxanthineen_US
dc.subjectRP-HPLCen_US
dc.subjectDADen_US
dc.subjectTheobromineen_US
dc.subjectTheophyllineen_US
dc.titleSimultaneous Determination of Theobromine, Paraxanthine, Theophylline, and Caffeine in Urine by Reversed-Phase High-Performance Liquid Chromatography with Diode Array UV Detectionen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu