Impact of ghrelin on vitreous cytokine levels in an experimental uveitis model
Küçük Resim Yok
Tarih
2013
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Dove Medical Press Ltd
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Background: The purpose of this study was to investigate the effect of intraperitoneal ghrelin on vitreous levels of interleukin (IL)-1, IL-6, and tumor necrosis factor-alpha (TNF-alpha) and to compare its effects with those of intraperitoneal infliximab in an experimental uveitis model. Methods: Twenty-four male rats were assigned to four groups of six rats in each. All the rats, except for those in group 1 (controls), were injected intravitreally with concanavalin A to induce experimental uveitis. Rats in group 2 (sham) were not given any treatment after uveitis was induced. Rats in group 3 were given intraperitoneal infliximab 0.5 mg/100 mL on days 0, 1, 3, 5, and 7 following induction of uveitis on day 14 of the study. Rats in group 4 were given intraperitoneal ghrelin 10 ng/kg/day for 7 days following induction of uveitis. On day 21 of the study, enucleated globes were subjected to histopathologic examination. Vitreous levels of IL-1, IL-6, and TNF-alpha were measured by enzyme-linked immunosorbent assay. Results: Vitreous levels of IL-1, IL-6, and TNF-alpha were significantly increased in the sham group relative to the control group (P < 0.05), but showed a significant decrease in the group treated with infliximab (P < 0.05). Cytokine levels also decreased in the ghrelin-treated group, but the decrease was not statistically significant (P > 0.05). Conclusion: Ghrelin failed to decrease the IL-1, IL-6, and TNF-alpha levels that play a critical role in the pathogenesis of uveitis.
Açıklama
Anahtar Kelimeler
uveitis, tumor necrosis factor-alpha, interleukin-1, interleukin-6, infliximab, ghrelin
Kaynak
Drug Design Development and Therapy
WoS Q Değeri
Q2
Scopus Q Değeri
Q1
Cilt
7
