Pokeweed (Phytolacca americana L.) antiviral protein inhibits Zucchini yellow mosaic virus infection in a dose-dependent manner in squash plants

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dc.authoridMühendislik Fakültesi, Harran Üniversitesi/0000-0001-7407-1635
dc.authoridOzcan, Dilek/0000-0002-0356-2936
dc.authoridPallas, Vicente/0000-0003-4954-989X
dc.authorwosidMühendislik Fakültesi, Harran Üniversitesi/GXH-7079-2022
dc.authorwosidNavarro, Jose/JQV-4305-2023
dc.authorwosidOzcan, Dilek/HHS-2303-2022
dc.authorwosidUsta, Mustafa/CAG-7521-2022
dc.authorwosidSipahioglu, Hikmet Murat/AAA-6085-2020
dc.authorwosidPallas, Vicente/N-1317-2014
dc.contributor.authorSipahioglu, Hikmet Murat
dc.contributor.authorKaya, Ilhan
dc.contributor.authorUsta, Mustafa
dc.contributor.authorUnal, Murat
dc.contributor.authorOzcan, Dilek
dc.contributor.authorOzer, Meryem
dc.contributor.authorGuller, Abdullah
dc.date.accessioned2024-08-04T20:09:52Z
dc.date.available2024-08-04T20:09:52Z
dc.date.issued2017
dc.departmentİnönü Üniversitesien_US
dc.description.abstractPokeweed antiviral protein (PAP) of Phytolacca americana L. (pokeweed) is a single-chain ribosome-inactivating protein (RIP) characterized by its ability to depurinate plant ribosomes. Here, we isolated, cloned, and expressed the ribosome inactivating protein (RIP) gene, designated as pokeweed antiviral protein type 1 (PAP I), from the summer leaves of pokeweed collected from the Black Sea region (Turkey). Our findings presented here provide direct evidence that exogenous application of PAP I causes concentration-dependent inhibition of Zucchini yellow mosaic virus (ZYMV) infection on squash plants. Squash plants were exposed to PAP I protein with and without DMSO for four consecutive days. Regular spraying of approximately 30 kDa recombinant PAP I at 2 mu g mL(-1) concentration prevented treated plants from mechanical virus infection. PAP I showed antiviral activity in 9 plants out of 15 inoculated plants. Remarkably, simultaneous application of PAP, DMSO, and ZYMV did not prevent virus infection, suggesting that PAP did not have any effect on viral RNA. In the absence of ZYMV the purified peptide was not cytotoxic for squash plants, although a reduction of plant size, possibly caused by host ribosome depurination, was observed.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [110 O 180]en_US
dc.description.sponsorshipFinancial support for this study was provided by the Scientific and Technological Research Council of Turkey (TUBITAK Project No: 110 O 180).en_US
dc.identifier.doi10.3906/tar-1612-30
dc.identifier.endpage262en_US
dc.identifier.issn1300-011X
dc.identifier.issn1303-6173
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-85029602655en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage256en_US
dc.identifier.trdizinid294985en_US
dc.identifier.urihttps://doi.org/10.3906/tar-1612-30
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/294985
dc.identifier.urihttps://hdl.handle.net/11616/92481
dc.identifier.volume41en_US
dc.identifier.wosWOS:000408334500002en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.publisherTubitak Scientific & Technological Research Council Turkeyen_US
dc.relation.ispartofTurkish Journal of Agriculture and Forestryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectPhytolacca americanaen_US
dc.subjectantiviral proteinen_US
dc.subjectexpressionen_US
dc.subjectinhibitionen_US
dc.subjectZucchini yellow mosaic virusen_US
dc.subjectsquashen_US
dc.titlePokeweed (Phytolacca americana L.) antiviral protein inhibits Zucchini yellow mosaic virus infection in a dose-dependent manner in squash plantsen_US
dc.typeArticleen_US

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