Assessment of antioxidant, acetylcholinesterase, paraoxonase inhibition activities and phenolic content of Alchemilla lithophila
Küçük Resim Yok
Tarih
2024
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Univ Karachi
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
In the present study, antioxidant activity and inhibition of acetylcholinesterase (AChE) and paraoxonase (hPON 1) of Alchemilla lithophila extracts were evaluated for the first time. Besides, there is no research on the contents of phenolic compounds except for fatty acids. In this context, phenolic compounds of A. lithophila were investigated by liquid chromatography/ mass spectrometry (LC-MS/MS). The methanol extract of the A. lithophila exhibited significant inhibition on the AChE (IC50 value for methanol extract 0.162 +/- 0.25 mg /mL, R-2 :0.992). Besides, antioxidant activities of the A. lithophila extracts were examined using by the methods ABTS(center dot+)and DPPH center dot free radical scavenging potentials, FRAP and CUPRAC metal-reducing activities. ABTS(center dot+) and DPPH center dot scavenging activities were found for methanol extract at 70.67% and water extract at 75.38%, respectively. Also, FRAP and CUPRAC metal-reducing were determined for water extract 0.796 and hexane extract 1.570 as absorbance. According to LC-MS/MS analyses, the amounts of ellagic acid, catechin hydrate, gallic acid, fumaric acid, luteolin, quercetin, kaempferol, acetohydroxamic acid, caffeic acid, syringic acid, hydroxybenzoic acid and salicylic acid were determined by LC-MS/MS, respectively. As a consequence, this study will be a useful resource for determining bioactivity and phenolic compound profile for natural medicine research.
Açıklama
Anahtar Kelimeler
Acetylcholinesterase, paraoxonase, antioxidant, Alchemilla lithophila, LC-MS/MS
Kaynak
Pakistan Journal of Pharmaceutical Sciences
WoS Q Değeri
N/A
Scopus Q Değeri
Q3
Cilt
37
Sayı
1
