Preparation of the L-Asparaginase-Based Biosensor With Polyimide Membrane Electrode for Monitoring L-Asparagine Levels in Leukemia
Küçük Resim Yok
Tarih
2014
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Taylor & Francis As
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Polyimide based amperometric biosensor was developed for the in vitro determination of L-asparagine in serum samples for monitoring L-asparagine levels in leukemia. First, polyimide from pyromellitic dianhydride and 4,4-oxydianiline (ODA) was prepared. This prepared polyimide was used as an immobilization membrane of L-asparaginase (L-ASNase) for L-ASNase-polyimide-coated electrodes. From the amperometric results for the polyimide electrode, it is concluded that polyimide membrane containing L-asparaginase can be used as a membrane for asparagine detection in the presence of large concentration of interferences, because of its strong adherence to the electrode surface, easy preparation, chemical stability, selectivity, and very high statistical confidence (R=0.9988).
Açıklama
Anahtar Kelimeler
Biosensor, immobilization, L-asparaginase, L-asparagine, polyimide membrane
Kaynak
International Journal of Polymeric Materials and Polymeric Biomaterials
WoS Q Değeri
Q1
Scopus Q Değeri
Q2
Cilt
63
Sayı
17
