Stafilokoklarda metisilin direncinin saptanmasında kullanılan yöntemlerin karşılaştırılması
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Dosyalar
Tarih
2002
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Mikrobiyoloji Bülteni
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Öz: Bu çalışmada, laboratuvarımızda rutin olarak uygulanan disk difüzyon yöntemiyle metisiline dirençli olarak saptanan 112 Staphylococcus aureus ve 93 koagülaz negatif stafilokok (KNS) suşunda metisilin direnci, mikrodilüsyon ve oksasilin tuz agar tarama testleri çalışılarak disk difüzyon yöntemiyle karşılaştırmıştır. Uyumsuz sonuçlarda polimeraz zincir reaksiyon yöntemiyle mecA geni araştırılmıştır. S.aureus suşlarının tamamı (%100) ile KNS suşunun %69.9'u (65/93) her üç yöntemle de metisiline dirençli olarak belirlenmiştir. Disk difüzyon yöntemiyle dirençli bulunan 28 KNS suşu işe, oksasilin tuz agar tarama yöntemiyle duyarlı olarak bulunmuş ve bu suşların 27'sinde mecA geni pozitif olarak saptanmıştır. Bu sonuçlar, S.aureus suşlarında metisilin direncini saptamada test edilen her üç yöntemin de uygun olduğunu, ancak KNS suşları için oksasilin tuz agar yönteminin yetersiz kaldığını göstermektedir.
Abstract: In this study, the methicillin resistance of 112 Staphylococcus aureus and 93 coagulase negative Staphylococcus (CNS) strains, which were initially found methicillin resistant by routine disk diffusion method in our laboratory, have been searched by microdilution and oxacillin agar screen test and the results were compared with the results obtained by disk diffusion method. The presence of mecA gene was investigated by polymerase chain reaction in case of discordant results. All S.aureus strains (100%) and 69.9% (65/93) of CNS strains were found resistant to methicillin by three of the methods. Of CNS isolates, 28 strains which were found methicillin resistant by disk diffusion method, were found methicillin susceptible by oxacillin agar screen method, and 27 of these were detected as mecA positive. Our results indicated that, the three methods tested were reliable for the detection of methicillin resistance in S.aureus strains, but oxacillin agar screen revealed to be unsatisfactory for the detection of methicillin resistance in CNS.
Abstract: In this study, the methicillin resistance of 112 Staphylococcus aureus and 93 coagulase negative Staphylococcus (CNS) strains, which were initially found methicillin resistant by routine disk diffusion method in our laboratory, have been searched by microdilution and oxacillin agar screen test and the results were compared with the results obtained by disk diffusion method. The presence of mecA gene was investigated by polymerase chain reaction in case of discordant results. All S.aureus strains (100%) and 69.9% (65/93) of CNS strains were found resistant to methicillin by three of the methods. Of CNS isolates, 28 strains which were found methicillin resistant by disk diffusion method, were found methicillin susceptible by oxacillin agar screen method, and 27 of these were detected as mecA positive. Our results indicated that, the three methods tested were reliable for the detection of methicillin resistance in S.aureus strains, but oxacillin agar screen revealed to be unsatisfactory for the detection of methicillin resistance in CNS.
Açıklama
Anahtar Kelimeler
Kaynak
Mikrobiyoloji Bülteni
WoS Q Değeri
Scopus Q Değeri
Cilt
36
Sayı
3-4
Künye
KUZUCU Ç,DALGALAR M,DURMAZ R,DİKEREL Ş (2002). Stafilokoklarda metisilin direncinin saptanmasında kullanılan yöntemlerin karşılaştırılması. Mikrobiyoloji Bülteni, 36(3-4), 253 - 257.
