Effect of humanine on the Notch signaling pathway in myocardial infarction

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dc.authoridkuloğlu, tuncay/0000-0001-9874-3838
dc.authoridÖZDEM, BERNA/0000-0002-4481-2866
dc.authorwosidonat, elif/Y-3938-2019
dc.authorwosidkuloğlu, tuncay/W-2310-2018
dc.authorwosidÖZDEM, BERNA/ACT-5099-2022
dc.contributor.authorOnat, Elif
dc.contributor.authorOnalan, Ebru
dc.contributor.authorOzdem, Berna
dc.contributor.authorBalgetir, Merve Kavak
dc.contributor.authorKuloglu, Tuncay
dc.date.accessioned2024-08-04T20:10:23Z
dc.date.available2024-08-04T20:10:23Z
dc.date.issued2023
dc.departmentİnönü Üniversitesien_US
dc.description.abstractBackground/aim: By applying humanin (HN) before myocardial infarction (MI), its protection in myocardial injury and the possible roles of its cellular mechanism in the Notch pathway were investigated. Materials and methods: The study was carried out at Firat University Experimental Research Center (12/24/2018-12/23/2019). Spraque-Dawley rats were divided into 10 groups: I (control) (n = 6), II (HN 6 h) (n = 6), III (HN 24 h) (n = 6), IV (HN day 7) (n = 6), V (MI 6 h) (n = 7), VI (MI 24 h) (n = 7), VII (MI day 7) (n = 7), VIII (MI+HN 6 h) (n = 7), IX (MI+HN 24 h) (n = 7), and X (MI+HN day 7) (n = 7). To create MI, 200 mg/kg of isoproterenol (ISO) was administered to the rats subcutaneously. Moreover, 252 mu g/kg of HN was given intraperitoneally (ip) to the rats on its own and before MI. Molecular parameters Notch1, Notch2, Hes1, Hes2, Jagged1, Jagged2, DLL1, and DLL4 were examined using polymerase chain reaction in the heart tissue, Notch1, Hes1, and DLL4 were examined using western blot, while heart tissue was taken for histochemical examinations. Results: The mRNA expression levels of the Notch signaling members (Notch1, Notch2, Hes1, Hes2, Jagged1, Jagged2, DLL1, and DLL4) tended to decrease after MI. The Notch signaling members increased more significantly, especially toward day 7 after HN application before MI. In the western blot anylyses, the Notch1, Hes1, and DLL4 protein levels increased significantly toward day 7 in the groups given HN before MI. Moreover, the serum AST, LDH, CK-MB, and troponin I levels tended to decrease with the application of HN before MI and there was a significant decrease in edema, hemorrhage, and mononuclear cells in the heart tissue at 24 h post-MI and fibrosis on day 7 post-MI. Conclusion: HN administration before MI has a cardioprotective effect on rats via the Notch signaling pathway.en_US
dc.description.sponsorshipScientific Research Grants Unit of Firat University [TF.18.60]en_US
dc.description.sponsorshipThe approval of the Animal Ethics Committee of Firat University Medical Faculty, Elazi & gbreve;, Turkiye was obtained for animal experiments (Protocol No.: 2018/91) . This study was funded by the Scientific Research Grants Unit of Firat University (grant number: TF.18.60) . We would like to acknowledge www.makaletercume.com for their outstanding statistics services that were provided for this manuscript. Also, we express our grateful thanks to Prof. Dr. Engin & Scedil;AHNA for his contributions.en_US
dc.identifier.doi10.55730/1300-0144.5734
dc.identifier.endpage1666en_US
dc.identifier.issn1300-0144
dc.identifier.issn1303-6165
dc.identifier.issue6en_US
dc.identifier.pmid38813496en_US
dc.identifier.scopus2-s2.0-85179910829en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage1658en_US
dc.identifier.trdizinid1221017en_US
dc.identifier.urihttps://doi.org/10.55730/1300-0144.5734
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/1221017
dc.identifier.urihttps://hdl.handle.net/11616/92757
dc.identifier.volume53en_US
dc.identifier.wosWOS:001127245500020en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTubitak Scientific & Technological Research Council Turkeyen_US
dc.relation.ispartofTurkish Journal of Medical Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectMyocardial infarctionen_US
dc.subjecthumaninen_US
dc.subjectNotch1en_US
dc.subjectHes1en_US
dc.subjectDll4en_US
dc.titleEffect of humanine on the Notch signaling pathway in myocardial infarctionen_US
dc.typeArticleen_US

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