The effects of phenoxybenzamine on tyrosine hydroxylase (TH) and TH mRNA level in adrenal medulla of sprague dawley rats
Küçük Resim Yok
Tarih
1998
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The effects of anti-hipertensive phenoxybenzamine were investigated on tyrosine hydroxylase (TH) enzyme activity and TH mRNA levels. In the present study 5 months male sprague dawley (SD) rats were used. Phenoxybenzamine was injected i.p. as 20 mg/kg which prepared in the 0.9% NaCl and 5% ethanole. TH activity was measured by detecting of formation of 3H2O as a formation of dopa from 3H-tyrosine by radioisotope technique total adrenal RNA was isolated and hybridized with 32P labeled cDNA. TH mRNA was assayed by densitometric scanning of the autoradiograms using a densitometer. TH activity and TH mRNA levels were found to be significantly increased by the effect of phenoxybenzamine (P<0.01).
Açıklama
Anahtar Kelimeler
Adrenal medulla, Phenoxybenzamine, TH mRNA, Tyrosine hydroxylase
Kaynak
Turkish Journal of Medical Sciences
WoS Q Değeri
Scopus Q Değeri
Q3
Cilt
28
Sayı
1
