Design of starch functionalized biodegradable P(MAA-co-MMA) as carrier matrix for L-asparaginase immobilization
Küçük Resim Yok
Tarih
2016
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier Sci Ltd
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
We prepared biodegradable P(MAA-co-MMA)-starch composite as carrier matrix for the immobilization of L-asparaginase (L-ASNase), an important chemotherapeutic agent in acute lymphoblastic leukemia. Chemical characteristics and thermal stability of the prepared composites were determined by FT-IR, TGA, DTA and, DSC, respectively. Also, biodegradability measurements of P(MAA-co-MMA)-starch composites were carried out to examine the effects of degradation of the starch. Then, L-ASNase was immobilized on the P(MAA-co-MMA)-starch composites. The surface morphology of the composite before and after immobilization was characterized by SEM, EDX, and AFM. The properties of the immobilized L-ASNase were investigated and compared with the free enzyme. The immobilized L-ASNase had better showed thermal and pH stability, and remained stable after 30 days of storage at 25 degrees C. Thus, based on the findings of the present work, the P(MAA-co-MMA)-starch composite can be exploited as the biocompatible matrix used for L-ASNase immobilization for medical applications due to biocompatibility and biodegradability. (C) 2016 Elsevier Ltd. All rights reserved.
Açıklama
Anahtar Kelimeler
L-Asparaginase, Enzyme immobilization, P(MAA-co-MMA), Starch, Biocomposite
Kaynak
Carbohydrate Polymers
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
153
