Facile construction of a robust and recyclable laccase/metal-organic framework-808 with boosted stability for biodegradation of Reactive Blue 171 and Reactive Blue 198 from aqueous media
Küçük Resim Yok
Tarih
2024
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Laccase enzyme sees application demand in many industries such as textile, food processing, wood processing, pharmaceutical, and chemical. However, it becomes difficult to maintain the enzymatic activity of laccase under harsh reaction conditions and even after several biocatalytic cycles. Therefore, suitably prescribed supports are crucial to ensure that the catalytic activity and reusability of the immobilized laccase are maintained at high levels. The present study highlights the development and dye decolorization application of an enzyme carrier matrix employing a Zr-based metal-organic framework (MOF), MOF-808, with hierarchical porosity for the immobilization of laccase from Trametes trogii . The immobilization efficiency for Lac/MOF-808 was found to be 70.83 +/- 1.98 %. Lac/MOF-808 exhibited optimal catalytic temperature at 60 degrees C, while the optimal catalytic pH was 4.5. Due to recoverable properties from the carrier matrix the recycle test was satisfactory for the Lac/MOF808, being reused 7 times without losses greater than 50 % of residual activity. Stored at 25 degrees C, Lac/MOF-808 retained 30 % of its activity during 4-weeks period. A kinetic study was carried out for Lac/MOF-808 monitoring the oxidation reaction of ABTS. The K-m value was 0.070 mM and the V-max was 87.7 mu mol min(-1) g(-1). Kinetic investigations, on the other hand, demonstrated a decrease in K m following immobilization, signifying a higher affinity for substrate. The Lac/MOF-808 maintained its decolorization efficiency even after eight reaction cycles, exhibiting a remarkable activity of 56.7 % and 77.2 % against Reactive Blue 171 and Reactive Blue 198, respectively. Hence, the described Lac/MOF-808 might be an ideal candidate for efficient dye decolorization applications.
Açıklama
Anahtar Kelimeler
Laccase, Trametes trogii, Enzyme immobilization, Metal-organic framework, Dye decolorization
Kaynak
Molecular Catalysis
WoS Q Değeri
N/A
Scopus Q Değeri
Q2
Cilt
562
