Facile construction of a robust and recyclable laccase/metal-organic framework-808 with boosted stability for biodegradation of Reactive Blue 171 and Reactive Blue 198 from aqueous media

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dc.authoridBoran, Filiz/0000-0002-8801-7987
dc.contributor.authorBakar, Busra
dc.contributor.authorBugday, Nesrin
dc.contributor.authorBirhanli, Emre
dc.contributor.authorBoran, Filiz
dc.contributor.authorUlu, Ahmet
dc.contributor.authorYasar, Sedat
dc.contributor.authorYesilada, Ozfer
dc.date.accessioned2024-08-04T20:56:00Z
dc.date.available2024-08-04T20:56:00Z
dc.date.issued2024
dc.departmentİnönü Üniversitesien_US
dc.description.abstractLaccase enzyme sees application demand in many industries such as textile, food processing, wood processing, pharmaceutical, and chemical. However, it becomes difficult to maintain the enzymatic activity of laccase under harsh reaction conditions and even after several biocatalytic cycles. Therefore, suitably prescribed supports are crucial to ensure that the catalytic activity and reusability of the immobilized laccase are maintained at high levels. The present study highlights the development and dye decolorization application of an enzyme carrier matrix employing a Zr-based metal-organic framework (MOF), MOF-808, with hierarchical porosity for the immobilization of laccase from Trametes trogii . The immobilization efficiency for Lac/MOF-808 was found to be 70.83 +/- 1.98 %. Lac/MOF-808 exhibited optimal catalytic temperature at 60 degrees C, while the optimal catalytic pH was 4.5. Due to recoverable properties from the carrier matrix the recycle test was satisfactory for the Lac/MOF808, being reused 7 times without losses greater than 50 % of residual activity. Stored at 25 degrees C, Lac/MOF-808 retained 30 % of its activity during 4-weeks period. A kinetic study was carried out for Lac/MOF-808 monitoring the oxidation reaction of ABTS. The K-m value was 0.070 mM and the V-max was 87.7 mu mol min(-1) g(-1). Kinetic investigations, on the other hand, demonstrated a decrease in K m following immobilization, signifying a higher affinity for substrate. The Lac/MOF-808 maintained its decolorization efficiency even after eight reaction cycles, exhibiting a remarkable activity of 56.7 % and 77.2 % against Reactive Blue 171 and Reactive Blue 198, respectively. Hence, the described Lac/MOF-808 might be an ideal candidate for efficient dye decolorization applications.en_US
dc.identifier.doi10.1016/j.mcat.2024.114203
dc.identifier.issn2468-8231
dc.identifier.scopus2-s2.0-85192840290en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1016/j.mcat.2024.114203
dc.identifier.urihttps://hdl.handle.net/11616/101991
dc.identifier.volume562en_US
dc.identifier.wosWOS:001241047500001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofMolecular Catalysisen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectLaccaseen_US
dc.subjectTrametes trogiien_US
dc.subjectEnzyme immobilizationen_US
dc.subjectMetal-organic frameworken_US
dc.subjectDye decolorizationen_US
dc.titleFacile construction of a robust and recyclable laccase/metal-organic framework-808 with boosted stability for biodegradation of Reactive Blue 171 and Reactive Blue 198 from aqueous mediaen_US
dc.typeArticleen_US

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