Influence of Lipoxin-A4 Treatment on Cytokine, Chemokine Genes Expression, and Phenotypic Distribution of Lymphocyte Subsets During Experimental Liver Fibrosis

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dc.authoridKAYHAN, BASAK/0000-0003-3508-2563
dc.authoridKURTOĞLU, Elçin Latife/0000-0002-8375-8399
dc.authoridKARACA, Zeynal Mete/0000-0002-5967-8102
dc.authorwosidKAYHAN, BASAK/ABH-9314-2020
dc.authorwosidKURTOĞLU, Elçin Latife/AAQ-6842-2020
dc.authorwosidKARACA, Zeynal Mete/HKM-7924-2023
dc.contributor.authorKaraca, Zeynal Mete
dc.contributor.authorKurtoglu, Elcin Latife
dc.contributor.authorGul, Mehmet
dc.contributor.authorKayhan, Basak
dc.date.accessioned2024-08-04T20:10:10Z
dc.date.available2024-08-04T20:10:10Z
dc.date.issued2022
dc.departmentİnönü Üniversitesien_US
dc.description.abstractObjective: Lipoxins are anti-inflammatory, pro-resolving molecules that are secreted by immune cells such as neutrophils and macrophages. Lipoxins are a metabolite of the arachidonic acid pathway that resolve inflammation in fibrotic liver by producing several anti-inflammatory molecules. In this study, phenotypic distribution activation markers of lymphocytes in the spleen and expression levels of chemokines (chemokine (C-X-C motif) receptor 3, chemokine (C-X-C motif) ligand 10) cytokines (interferon gamma, tumor necrosis factor alpha, interleukin-6, interleukin-10) in the liver of lipoxin A4-treated fibrotic mice were investigated. Materials and methods: Liver fibrosis was induced in BALB/c mice by thioacetamide administration. Lipoxin A4 was administered during last 2 weeks of induction. Fibrosis level was determined by using Knodell scoring. Lymphocytes were identified by flow-cytometry. Expression levels of genes were measured by quantitative real time polymerase chain reaction in liver homogenates. Results: Lipoxin A4 treatment caused an elevation of T-lymphocyte percentage in the spleen. Interestingly, administration of lipoxin A4 significantly reduced B-lymphocyte population in spleen of fibrotic group. CD8(+) cytotoxic T cell frequency significantly reduced in thioacetamide-induced mice; however, lipoxin A4 administration increased that percentage significantly. Lipoxin A4 treatment significantly reduced frequency of activated (CD8(+)CD69(+)) cytotoxic T cells. Expression levels of chemokines significantly reduced in the liver after lipoxin A4 treatment. While expression levels of interferon gamma, tumor necrosis factor alpha. and interleukin-6 significantly reduced in the liver after lipoxin A4 treatment, an anti-inflammatory cytokine interleukin-10 expression was almost at similar levels in all experimental groups. Conclusion: Lipoxin A4 performs its anti-inflammatory effect by reducing the frequency of activated T cells and expression levels of chemokines cytokines responsible from inflammatory immune response in the liver.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey; Scientific Research Projects Coordination Unit of Inonu University [TYL-2018-1430]en_US
dc.description.sponsorshipThat study has been supported by The Scientific and Technological Research Council of Turkey with project number114S161/1002; Scientific Research Projects Coordination Unit of Inonu University with project number TYL-2018-1430.en_US
dc.identifier.doi10.5152/eurasianjmed.2022.20030
dc.identifier.endpage35en_US
dc.identifier.issn1308-8742
dc.identifier.issue1en_US
dc.identifier.pmid35307625en_US
dc.identifier.scopus2-s2.0-85125866966en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage27en_US
dc.identifier.trdizinid524250en_US
dc.identifier.urihttps://doi.org/10.5152/eurasianjmed.2022.20030
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/524250
dc.identifier.urihttps://hdl.handle.net/11616/92632
dc.identifier.volume54en_US
dc.identifier.wosWOS:000776956600006en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherAvesen_US
dc.relation.ispartofEurasian Journal of Medicineen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectImmune responseen_US
dc.subjectlipoxin-A4en_US
dc.subjectliver fibrosisen_US
dc.titleInfluence of Lipoxin-A4 Treatment on Cytokine, Chemokine Genes Expression, and Phenotypic Distribution of Lymphocyte Subsets During Experimental Liver Fibrosisen_US
dc.typeArticleen_US

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