Occurrence and molecular characterization of acute bee paralysis virus (ABPV) in honeybee (Apis mellifera) colonies in Hakkari province
Küçük Resim Yok
Tarih
2016
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Centenary University
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
A study was carried out during May-August 2014 to investigate the occurrence and prevalence of Acute bee paralysis virus (ABPV) in 90 different apiaries located in Hakkari province (Turkey). To detect the viral nucleic acids, an RT-PCR assay has been implemented for a sensitive and specific diagnosis of ABPV, by using a pair of primer specifically designed to coat protein gene of ABPV, amplifying a 476 bp nucleotide fragment. ABPV was found to occur in 2 samples from two distinct locations among 90 apiaries indicating that ABPV is not widespread in Hakkari province. To understand the molecular relationship between the isolates and the strains of the same virus, the amplified ABPV fragments cloned and sequenced and the sequence was compared with the fragments of ABPV sequences from different geographic origin of the world. The phylogenetic analysis revealed nucleotide identity rates between 85 and 97 %. The partial CP gene of ABPV sequence deposited in the GenBank database with the accession number of KP259311. © 2016, Centenary University. All rights reserved.
Açıklama
Anahtar Kelimeler
Acute bee paralysis virus, Hakkari province, Molecular characterization, Survey
Kaynak
Yuzuncu Yil University Journal of Agricultural Sciences
WoS Q Değeri
Scopus Q Değeri
Q3
Cilt
26
Sayı
2
