The Effects to GM-CSF Expression and Fibroblast Proliferation of pGM-CSF Containing Chitosan/PVP Hydrogels

Basit Öğe Kaydı
dc.authoridŞALVA, EMINE/0000-0002-1159-5850
dc.authoridSalva, Emine/0000-0002-1159-5850
dc.authorwosidŞALVA, EMINE/CAH-3062-2022
dc.authorwosidSalva, Emine/ABI-2766-2020
dc.contributor.authorSalva, Emine
dc.contributor.authorAkbuga, Julide
dc.date.accessioned2024-08-04T20:09:50Z
dc.date.available2024-08-04T20:09:50Z
dc.date.issued2017
dc.departmentİnönü Üniversitesien_US
dc.description.abstractThe aim of this study was to develop pGM-CSF containing chitosan/PVP hydrogel system for gene delivery. The hydrogels in semi-interpenetrating polymer network (semi-IPN) structure were synthesized by glutaraldehyde crosslinking between 2% chitosan and 2%, % 4, % 6 PVP. The viscosity, swelling properties and surface morphology of hydrogels were examined. These hydrogels showed highly porous structure. The porosity was increased with enhanced PVP concentration. Viscosity decreased with increasing PVP concentration of the hydrogels. The swelling percentage increased with increasing PVP concentration. In order to examine the effect of cell attachment and proliferation of hydrogels, NIH-3T3 fibroblast cell was used. The cells were able to attach on the top surface of hydrogels. However, the attachment of cells were slow at days 1 and 3. At days 7 and 14, cells looked healthy and normal and proliferated around and top of the hydrogels. With the ELISA study, the highest GM-CSF expression was obtained with pGM-CSF containing 2% chitosan+4% PVP hydrogels. MTT assay showed that chitosan/PVP hydrogels did not induce significant cytotoxic effect. As a result, these hydrogels could be served candidate for local gene delivery in tissue regeneration.en_US
dc.identifier.doi10.12991/marupj.278854
dc.identifier.endpage234en_US
dc.identifier.issn1309-0801
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-85014119655en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage228en_US
dc.identifier.trdizinid302692en_US
dc.identifier.urihttps://doi.org/10.12991/marupj.278854
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/302692
dc.identifier.urihttps://hdl.handle.net/11616/92472
dc.identifier.volume21en_US
dc.identifier.wosWOS:000411289700004en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.publisherMarmara Univ, Fac Pharmacyen_US
dc.relation.ispartofMarmara Pharmaceutical Journalen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectpGM-CSFen_US
dc.subjectchitosanen_US
dc.subjectPVPen_US
dc.subjecthydrogelen_US
dc.subjectgene deliveryen_US
dc.titleThe Effects to GM-CSF Expression and Fibroblast Proliferation of pGM-CSF Containing Chitosan/PVP Hydrogelsen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu
TR-Dizin İndeksli Yayınlar Koleksiyonu