Protective and therapeutic effect of apocynin on bleomycin induced lung fibrosis in rats
Küçük Resim Yok
Tarih
2015
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Inflammation
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
We aimed to investigate the preventive and therapeutic effect of apocynin (APO) on
bleomycin (BLC)-induced lung injury in rats. Rats were assigned into groups as follows: control group;
APO group, 20 mg/kg APO was given intraperitoneal for 29 days; BLC-1 and BLC-2 groups, a single
intratracheal injection of BLC (2.5 mg/kg); APO+BLC-preventive group, 20 mg/kg APO was administered
12 h before the intratracheal BLC injection and continued for 14 days; BLC+APO-treatment
group, 20 mg/kg APO was given on the 14th day after the intratracheal BLC injection and continued to
sacrifice. The BLC-1 group was sacrificed on the 14th day of BLC administration to validate BLCinduced
lung inflammation and fibrosis on the 14th of study initiation. All other groups were sacrificed
on the 29th day after BLC administration. The semiquantitative histopathological assessment, tissue
levels of malondialdehyde (MDA), superoxide dismutase, catalase (CAT), glutathione peroxidase
(GPx), reduced glutathione (GSH), total antioxidant capacity, total oxidant status (TOS), and oxidative
stress index (OSI) were measured. An addition to the serum myeloperoxidase (MPO), the cell count and
cytokines (IL-1β, IL-6, and IL-8) of bronchoalveolar lavage (BAL) fluid were assayed. BLC-provoked
histological changes were significantly detected compared to the control group. APO restored these
histological damages in different quantity in the treatment and prevention groups. BLC caused a
significant decrease in GSH, CAT, and GPX, which were accompanied with significantly the increased
MDA, TOS levels, and OSI in the lung tissue concomitant with increased levels of the cellular account
and proinflammatory cytokines in the BAL fluid. Otherwise, APO administration, both before and after
BLC, reversed all biochemical markers and cytokine as well as histopathological changes induced by
BLC. Interestingly, APO treatment reversed MPO activity in serum increased by BLC. In this study, both
protective and therapeutic effects of APO against BLC-induced lung fibrosis were demonstrated for the
first time.
Açıklama
Anahtar Kelimeler
Apocynin, Antioxidant, Bleomycin, Cytokine, Pulmonary fibrosis
Kaynak
Inflammation
WoS Q Değeri
Scopus Q Değeri
Cilt
38
Sayı
3
Künye
Kılıç, T. Parlakpınar, H. Taşlıdere, E. Yıldız, S. Polat, A. Vardı, N. Çolak, C. Ermiş, H. (2015). Protective and therapeutic effect of apocynin on bleomycin induced lung fibrosis in rats. Inflammation. 38, (3); 1166-1180.
