Protective and therapeutic effect of apocynin on bleomycin induced lung fibrosis in rats

Basit Öğe Kaydı
dc.authorid9217en_US
dc.contributor.authorKılıç, Talat
dc.contributor.authorParlakpınar, Hakan
dc.contributor.authorTaşlıdere, Elif
dc.contributor.authorYıldız, Sedat
dc.contributor.authorPolat, Alaadin
dc.contributor.authorVardı, Nigar
dc.contributor.authorÇolak, Cemil
dc.contributor.authorErmiş, Hilal
dc.date.accessioned2017-08-18T06:44:10Z
dc.date.available2017-08-18T06:44:10Z
dc.date.issued2015
dc.departmentİnönü Üniversitesien_US
dc.description.abstractWe aimed to investigate the preventive and therapeutic effect of apocynin (APO) on bleomycin (BLC)-induced lung injury in rats. Rats were assigned into groups as follows: control group; APO group, 20 mg/kg APO was given intraperitoneal for 29 days; BLC-1 and BLC-2 groups, a single intratracheal injection of BLC (2.5 mg/kg); APO+BLC-preventive group, 20 mg/kg APO was administered 12 h before the intratracheal BLC injection and continued for 14 days; BLC+APO-treatment group, 20 mg/kg APO was given on the 14th day after the intratracheal BLC injection and continued to sacrifice. The BLC-1 group was sacrificed on the 14th day of BLC administration to validate BLCinduced lung inflammation and fibrosis on the 14th of study initiation. All other groups were sacrificed on the 29th day after BLC administration. The semiquantitative histopathological assessment, tissue levels of malondialdehyde (MDA), superoxide dismutase, catalase (CAT), glutathione peroxidase (GPx), reduced glutathione (GSH), total antioxidant capacity, total oxidant status (TOS), and oxidative stress index (OSI) were measured. An addition to the serum myeloperoxidase (MPO), the cell count and cytokines (IL-1β, IL-6, and IL-8) of bronchoalveolar lavage (BAL) fluid were assayed. BLC-provoked histological changes were significantly detected compared to the control group. APO restored these histological damages in different quantity in the treatment and prevention groups. BLC caused a significant decrease in GSH, CAT, and GPX, which were accompanied with significantly the increased MDA, TOS levels, and OSI in the lung tissue concomitant with increased levels of the cellular account and proinflammatory cytokines in the BAL fluid. Otherwise, APO administration, both before and after BLC, reversed all biochemical markers and cytokine as well as histopathological changes induced by BLC. Interestingly, APO treatment reversed MPO activity in serum increased by BLC. In this study, both protective and therapeutic effects of APO against BLC-induced lung fibrosis were demonstrated for the first time.en_US
dc.identifier.citationKılıç, T. Parlakpınar, H. Taşlıdere, E. Yıldız, S. Polat, A. Vardı, N. Çolak, C. Ermiş, H. (2015). Protective and therapeutic effect of apocynin on bleomycin induced lung fibrosis in rats. Inflammation. 38, (3); 1166-1180.en_US
dc.identifier.doi10.1007/s10753-014-0081-1en_US
dc.identifier.endpage1180en_US
dc.identifier.issue3en_US
dc.identifier.startpage1166en_US
dc.identifier.urihttps://hdl.handle.net/11616/7615
dc.identifier.volume38en_US
dc.language.isoenen_US
dc.publisherInflammationen_US
dc.relation.ispartofInflammationen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectApocyninen_US
dc.subjectAntioxidanten_US
dc.subjectBleomycinen_US
dc.subjectCytokineen_US
dc.subjectPulmonary fibrosisen_US
dc.titleProtective and therapeutic effect of apocynin on bleomycin induced lung fibrosis in ratsen_US
dc.typeArticleen_US

Dosyalar

Lisans paketi
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
license.txt
Boyut:
1.71 KB
Biçim:
Item-specific license agreed upon to submission
Açıklama:
İndir

Koleksiyon

Tıp Fakültesi, Biyoistatistik ve Tıp Bilişimi Anabilim Dalı Koleksiyonu