Effect of nitric oxide on bacterial translocation in experimental intestinal obstruction
Küçük Resim Yok
Tarih
2001
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Turkish Surgical Society
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Bacterial translocation is defined as the passage of endogenous bacteria from the gastrointestinal tract to the mesenteric lymph nodes and other organs that suggested to be the cause of sepsis in patient with multiple organ failure. The aim of this study was to investigate the antibacterial effect of nitric oxide by using nitric oxide synthetase enzyme inhibitors such as L-NAME and aminoguanidin in the obstructive intestinal rat model. Fourty rats were divided into four groups. The first one was control group and had only laparatomy. Intestinal obstruction was performed to second group, intestinal obstruction + L-NAME (L-NG-nitro-L-arginine methyl ester) were applied to third group. Fourth group had intestinal obstruction and aminoguanidin was injected to them. According to the histopathological study of the intestine, group III demonstrated less ulceration than groups II and IV (p<0.05). Mucosal damage and bacterial translocation showed no correlation according to the results. The incidence of bacterial translocation was 46.6% in group II, 73.3% in group III and 90% in group IV. Eighty two percent of isolated bacteria was typical enteric Gram negative organisms. Bacterial translocation was not observed in control group. Plasma nitric oxide levels of groups were 14.0±3.3, 31.2±7.6, 12.2±2.5, and 10.5±1.8 ?mol/L, respectively. As a conclusion; nitric oxide can damage mucosal integrity that may increase bacterial translocation but the antibacterial effect of nitric oxide may be more effective and nitric oxide can decrease the translocation of bacteria from gut.
Açıklama
Anahtar Kelimeler
Bacterial translocation, Intestinal obstruction, Nitric oxide
Kaynak
Turkish Journal of Surgery
WoS Q Değeri
Scopus Q Değeri
Q3
Cilt
17
Sayı
4
