Genotoxic effects of tacrolimus on human lymphocyte cells
Küçük Resim Yok
Tarih
2012
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Maik Nauka/Interperiodica/Springer
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
We designed in vitro study to determine possible genotoxic effects of tacrolimus (FK-506), which is used as a potent immunosuppressive drug, by using sister chromatid exchange (SCEs), chromosome aberration (CAs), micronuclei tests (MN) and cell growth kinetics such as mitotic index (MI) and replication index (RI) in human lymphocytes. The ceils were treated with 5, 25, 50, and 100 ng/ml concentrations of tacrolimus, for 24 and 48 h treatment periods. Tacrolimus induced CA and MN frequency at all concentrations for 24 and 48 h. In additon, it induced the SCE at the highest concentration for 24 h and at 25 and 100 ng/ml for 48 h. Tacrolimus decreased MI at all concentrations (except 5 ng/ml) for all treatment periods. It also inhibited the RI at 50 and 100 ng/ml concentrations for 24 h and at all concentrations for 48 h. Treatments given with tacrolimus result in the enhance of the different endpoints of genotoxicity, suggesting its mutagenic action on lymphocytes in vitro.
Açıklama
Anahtar Kelimeler
Renal-Transplantation, Chromosomal-Aberrations, Immunosuppressive Drugs, Cyclosporine-A, Fk-506, Mutagenicity, Carcinogens, Recipients, Cancer, Assay
Kaynak
Russian Journal of Genetics
WoS Q Değeri
Q4
Scopus Q Değeri
Q4
Cilt
48
Sayı
6
