Design of highly selective, and sensitive screen-printed electrochemical sensor for detection of uric acid with uricase immobilized polycaprolactone/polyethylene imine electrospun nanofiber
Küçük Resim Yok
Tarih
2023
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Pergamon-Elsevier Science Ltd
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Uric acid (UA) plays a significant role in nerve center, human metabolism, and kidney system. Therefore, it is necessary to establish a simple, rapid, and sensitive detection method for UA. In recent years, researchers have been highly attracted by nanomaterials with satisfactory functions in electrochemical applications. In this study, polycaprolactone (PCL)/polyethylene imine (PEI) nanofiber membranes were prepared and used for the immobilization of uricase (UOx). The prepared membranes were characterized in terms of structure, composi-tion, and morphology. Afterward, the quantum dot screen printed electrode (QD SPCE) was modified with PCL/ PEI nanofiber membranes with and without methylene blue (MB) as an electron mediator. The electrochemical performance of the developed sensors was investigated by cyclic voltammetry (CV), differential pulse voltam-metry (DPV), and electrochemical impedance spectroscopy (EIS). Under the optimal conditions, the modified sensors provided a broad linear range (5.0-52.0 mu M) for the electrochemical detection of UA. Limit of detection (LoD) values were determined as 3.96 mu M and 1.85 mu M for the PCL/PEI/UOx/QD SPCE and PCL/PEI/UOx/MB/ QD SPCE, respectively. The four-week stability results showed the change in current for the PCL/PEI/UOx/QD SPCE and PCL/PEI/UOx/MB/QD SPCE to be 92% and 87%, respectively. Additionally, in the mixed interference test remaining current ratios for UA were 95% and 82% for the PCL/PEI/UOx/QD SPCE and PCL/PEI/UOx/MB/ QD SPCE, respectively. Most importantly, the effectiveness of the electrodes was also verified in real sample detection with satisfactory recovery (similar to 98-112%). Overall, the results showed that the PCL/PEI/UOx/QD SPCE and PCL/PEI/UOx/MB/QD SPCE have the advantages of simplicity, high sensitivity, and good selectivity for UA determination.
Açıklama
Anahtar Kelimeler
Electrospun nanofiber, Uricase, Enzyme immobilization, Quantum dot screen printed electrode, Differential pulse voltammetry, Uric acid, Biosensor
Kaynak
Electrochimica Acta
WoS Q Değeri
Q2
Scopus Q Değeri
Q1
Cilt
439
