Design of highly selective, and sensitive screen-printed electrochemical sensor for detection of uric acid with uricase immobilized polycaprolactone/polyethylene imine electrospun nanofiber

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dc.authoridUlu, Ahmet/0000-0002-4447-6233
dc.authoridMuhammad, Fakhriy/0000-0001-8417-5075
dc.authorwosidUlu, Ahmet/L-5180-2016
dc.contributor.authorMuhammad, Fakhriy
dc.contributor.authorDik, Gamze
dc.contributor.authorKolak, Seda
dc.contributor.authorGedik, Kubra Karadas
dc.contributor.authorBakar, Busra
dc.contributor.authorUlu, Ahmet
dc.contributor.authorAtes, Burhan
dc.date.accessioned2024-08-04T20:53:16Z
dc.date.available2024-08-04T20:53:16Z
dc.date.issued2023
dc.departmentİnönü Üniversitesien_US
dc.description.abstractUric acid (UA) plays a significant role in nerve center, human metabolism, and kidney system. Therefore, it is necessary to establish a simple, rapid, and sensitive detection method for UA. In recent years, researchers have been highly attracted by nanomaterials with satisfactory functions in electrochemical applications. In this study, polycaprolactone (PCL)/polyethylene imine (PEI) nanofiber membranes were prepared and used for the immobilization of uricase (UOx). The prepared membranes were characterized in terms of structure, composi-tion, and morphology. Afterward, the quantum dot screen printed electrode (QD SPCE) was modified with PCL/ PEI nanofiber membranes with and without methylene blue (MB) as an electron mediator. The electrochemical performance of the developed sensors was investigated by cyclic voltammetry (CV), differential pulse voltam-metry (DPV), and electrochemical impedance spectroscopy (EIS). Under the optimal conditions, the modified sensors provided a broad linear range (5.0-52.0 mu M) for the electrochemical detection of UA. Limit of detection (LoD) values were determined as 3.96 mu M and 1.85 mu M for the PCL/PEI/UOx/QD SPCE and PCL/PEI/UOx/MB/ QD SPCE, respectively. The four-week stability results showed the change in current for the PCL/PEI/UOx/QD SPCE and PCL/PEI/UOx/MB/QD SPCE to be 92% and 87%, respectively. Additionally, in the mixed interference test remaining current ratios for UA were 95% and 82% for the PCL/PEI/UOx/QD SPCE and PCL/PEI/UOx/MB/ QD SPCE, respectively. Most importantly, the effectiveness of the electrodes was also verified in real sample detection with satisfactory recovery (similar to 98-112%). Overall, the results showed that the PCL/PEI/UOx/QD SPCE and PCL/PEI/UOx/MB/QD SPCE have the advantages of simplicity, high sensitivity, and good selectivity for UA determination.en_US
dc.description.sponsorshipUniversity Scientific Research Projects Unit; [FYL-2021-2567]; [FBG-2020-2173]en_US
dc.description.sponsorshipThis work was supported by the I ? no ? n? University Scientific Research Projects Unit (Project numbers: FYL-2021-2567 and FBG-2020-2173) .en_US
dc.identifier.doi10.1016/j.electacta.2022.141675
dc.identifier.issn0013-4686
dc.identifier.issn1873-3859
dc.identifier.scopus2-s2.0-85144416656en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1016/j.electacta.2022.141675
dc.identifier.urihttps://hdl.handle.net/11616/101071
dc.identifier.volume439en_US
dc.identifier.wosWOS:000910006700001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherPergamon-Elsevier Science Ltden_US
dc.relation.ispartofElectrochimica Actaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectElectrospun nanofiberen_US
dc.subjectUricaseen_US
dc.subjectEnzyme immobilizationen_US
dc.subjectQuantum dot screen printed electrodeen_US
dc.subjectDifferential pulse voltammetryen_US
dc.subjectUric aciden_US
dc.subjectBiosensoren_US
dc.titleDesign of highly selective, and sensitive screen-printed electrochemical sensor for detection of uric acid with uricase immobilized polycaprolactone/polyethylene imine electrospun nanofiberen_US
dc.typeArticleen_US

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