Primary drug resistance and molecular epidemiology of the Mycobacterium tuberculosis strains isolated in the Kelkit valley
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Dosyalar
Tarih
2009
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Turkish Journal of Medical Sciences
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Öz: Amaç: Bu çalışmada; Kelkit vadisinden izole edilen Mycobacterium tuberculosis izolatlarında primer ilaç direncinin ve direnç genotiplerinin belirlenmesi amaçlanmıştır. Yöntem ve Gereç: İzolatların, izoniazid, rifampisin, etambutol ve streptomisin dirençleri BACTEC metoduyla belirlendi. Kırk sekiz dirençli Mycobacterium tuberculosis suşu spoligotiplendirme yöntemiyle genotiplendirildi. Bulgular: En az bir ilaca direnç % 11.6, birden fazla ilaca direnç % 8.25 olarak belirlendi. Tek ilaç direnci sırası ile izoniazid (INH), etambutol (ETB), streptomisin (SM) ve rifampisin (RIF) için % 5.8, % 2.07, % 3.73 ve % 0 bulundu. İki veya daha fazla ilaca direnç oranları; streptomisin + etambutol, streptomisin + izoniazid, izoniazid + rifampisin, rifampisin + etambutol, izoniazid + etambutol + rifampisin, streptomisin + etambutol + izoniazid ve izoniazid + etambutol + rifampisin + streptomisin için sırasıyla; % 0.82, % 1.65, % 0.41, % 0.82, % 2.90, % 0.41 ve % 1.24 olarak belirlendi. Spoligotiplendirme sonuçlarına göre 15 farklı genotip paterni elde edildi. Bu yöntemle 4 izolat tiplendirilemedi. Çalışmamızda en sık rastlanan spoligotip kümeleri sırası ile ST4 (n = 10, % 20.8), ST53 (n = 6, % 12.5), ST7 (n = 4, % 8.3), ST41 (n = 4, % 8.3), ST31 (n = 4, % 8.3); ve familyaları TI (n = 18, % 37.5), S (n = 12, % 25) ve LAM-7 TUR (n = 4, % 8.3) olarak saptandı. Sonuç: Kelkit vadisinde yapılan bu ilk çalışmada; major anti tüberküloz ilaçlara karşı yüksek oranda direnç varlığı gözlendi.
Abstract: Aim: The aim of this study was to determine the primary drug resistance rates and genotypes of resistant Mycobacterium tuberculosis strains isolated in the Kelkit Valley. Materials and Methods: Primary resistance to isoniazid (INH), rifampicin (RIF), ethambutol (ETB), and streptomycin (SM) was determined with the BACTEC 460 radiometric system. Forty-eight resistant M. tuberculosis strains isolated from tuberculosis patients living in the Kelkit Valley were genotyped using the spoligotyping method. Results: Approximately 11.6% of the strains were resistant against at least one major drug, and 8.3% were resistant against more than one drug. Single-drug resistance for INH, ETB, SM, and RIF was found as 5.8%, 2.1%, 3.7%, and 0.0%, respectively. Two or more drug resistance rates for SM + ETB, SM + INH, INH + RIF, RIF + ETB, INH + ETB + RIF, SM + ETB + INH and INH + ETB + RIF + SM were determined as 0.8%, 1.7%, 0.4%, 0.8%, 2.9%, 0.4%, and 1.2%, respectively. Fifteen spoligotype groups were created when the results were compared with the world databank (SpoIDB4). Families of four strains could not be determined. The spoligotype groups most frequently encountered in our study were ST4 (n = 10, 20.8%), ST53 (n = 6, 12.5%), ST7 (n = 4, 8.3%), ST41 (n = 4, 8.3%) and ST31 (n = 4, 8.3%), and the most frequently encountered families were TI (n = 18, 37.5%), S (n = 12, 25%) and LAM-7 TUR (n = 4, 8.3%). Conclusions: High rates of primary resistance against major anti-tuberculosis drugs, the LAM-7 TUR clone, and high grouping rates were detected in this study, the first such study carried out in the Kelkit Valley.
Abstract: Aim: The aim of this study was to determine the primary drug resistance rates and genotypes of resistant Mycobacterium tuberculosis strains isolated in the Kelkit Valley. Materials and Methods: Primary resistance to isoniazid (INH), rifampicin (RIF), ethambutol (ETB), and streptomycin (SM) was determined with the BACTEC 460 radiometric system. Forty-eight resistant M. tuberculosis strains isolated from tuberculosis patients living in the Kelkit Valley were genotyped using the spoligotyping method. Results: Approximately 11.6% of the strains were resistant against at least one major drug, and 8.3% were resistant against more than one drug. Single-drug resistance for INH, ETB, SM, and RIF was found as 5.8%, 2.1%, 3.7%, and 0.0%, respectively. Two or more drug resistance rates for SM + ETB, SM + INH, INH + RIF, RIF + ETB, INH + ETB + RIF, SM + ETB + INH and INH + ETB + RIF + SM were determined as 0.8%, 1.7%, 0.4%, 0.8%, 2.9%, 0.4%, and 1.2%, respectively. Fifteen spoligotype groups were created when the results were compared with the world databank (SpoIDB4). Families of four strains could not be determined. The spoligotype groups most frequently encountered in our study were ST4 (n = 10, 20.8%), ST53 (n = 6, 12.5%), ST7 (n = 4, 8.3%), ST41 (n = 4, 8.3%) and ST31 (n = 4, 8.3%), and the most frequently encountered families were TI (n = 18, 37.5%), S (n = 12, 25%) and LAM-7 TUR (n = 4, 8.3%). Conclusions: High rates of primary resistance against major anti-tuberculosis drugs, the LAM-7 TUR clone, and high grouping rates were detected in this study, the first such study carried out in the Kelkit Valley.
Açıklama
Yıl: 2009Cilt: 39Sayı: 1ISSN: 1300-0144 / 1303-6165Sayfa Aralığı: 101 - 107Metin Dili:İngilizce
Anahtar Kelimeler
Kaynak
Turkish Journal of Medical Sciences
WoS Q Değeri
Scopus Q Değeri
Q3
Cilt
39
Sayı
1
Künye
BULUT Y,YENİŞEHİRLİ G,OTLU B,SEYFİKLİ Z,ÇELİKEL S,YILMAZ A,KÖSEOĞLU H. İ (2009). Primary drug resistance and molecular epidemiology of the Mycobacterium tuberculosis strains isolated in the Kelkit valley. Turkish Journal of Medical Sciences, 39(1), 101 - 107.
