HPLC method development and validation: Simultaneous determination of active ingredients in cough and cold pharmaceuticals
Küçük Resim Yok
Tarih
2014
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
International Journal of Pharmacy and Pharmaceutical Science
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Objective: This study aimed to develope a simple reversed-phase high performance chromatographic method for simultaneous determination of pseudoephdrine HCI, pheniramine maleate, acetaminophen, guaifenisin, pyrilamine maleate, chlorpheniramine maleate, triprolidine HCI, dextromethorphan HBr, diphenhydramine HCI in cough and cold pharmaceuticals.Methods: The separation of these compounds was achieved within 37.9 min on a Nucleodur gravity C18 column (250 × 4.0 mm, 5?m). The chromatographic separation of these compounds performed in a single run by using isocratic mobile phase consisting of methanol:buffer mixture (38:62, v/v) at room temperature, with flow rate of 0.75 mL.min-1. An ultraviolet absorption at 210 nm was monitored. 2,4,6-trimethoxybenzaldehyde was used as an internal standard (ISTD). The selectivity, linearity of calibration, accuracy, intraday and interday precision and forced degradation studies were examined as parts of the method validation.Results: The concentration–response relationship was linear over a concentration range of 0.2-250 ?g.mL-1for acetaminophen, 0.5–250 ?g.mL-1for pseudoephdrine HCI and pheniramine maleate, 1–250 ?g.mL-1for guaifenisin, 2.5-250 ?g.mL-1for chlorpheniramine maleate and triprolidine HCI, 5-250 ?g.mL-1for pyrilamine maleate and diphenhydramine HCI, 10-20 ?g.mL-1for dextromethorphan HBr with correlation coefficients better than 0.9993. The relative standard deviations of the intraday and interday were all less than 4%. Conclusion: The proposed liquid chromatographic method was successfully applied for the routine analysis of these compounds in different cough and cold pharmaceutical preparations such as syrups and tablets. © 2014, International Journal of Pharmacy and Pharmaceutical Science. All rights reserved.
Açıklama
Anahtar Kelimeler
Active ingredients cough and cold pharmaceuticals, High performance liquid chromatography, Validation
Kaynak
International Journal of Pharmacy and Pharmaceutical Sciences
WoS Q Değeri
Scopus Q Değeri
N/A
Cilt
6
Sayı
10
