HPLC method development and validation: Simultaneous determination of active ingredients in cough and cold pharmaceuticals

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dc.authorscopusid56397890300
dc.authorscopusid6603095173
dc.contributor.authorÇağlar H.
dc.contributor.authorBüyüktuncel E.
dc.date.accessioned2024-08-04T19:59:39Z
dc.date.available2024-08-04T19:59:39Z
dc.date.issued2014
dc.departmentİnönü Üniversitesien_US
dc.description.abstractObjective: This study aimed to develope a simple reversed-phase high performance chromatographic method for simultaneous determination of pseudoephdrine HCI, pheniramine maleate, acetaminophen, guaifenisin, pyrilamine maleate, chlorpheniramine maleate, triprolidine HCI, dextromethorphan HBr, diphenhydramine HCI in cough and cold pharmaceuticals.Methods: The separation of these compounds was achieved within 37.9 min on a Nucleodur gravity C18 column (250 × 4.0 mm, 5?m). The chromatographic separation of these compounds performed in a single run by using isocratic mobile phase consisting of methanol:buffer mixture (38:62, v/v) at room temperature, with flow rate of 0.75 mL.min-1. An ultraviolet absorption at 210 nm was monitored. 2,4,6-trimethoxybenzaldehyde was used as an internal standard (ISTD). The selectivity, linearity of calibration, accuracy, intraday and interday precision and forced degradation studies were examined as parts of the method validation.Results: The concentration–response relationship was linear over a concentration range of 0.2-250 ?g.mL-1for acetaminophen, 0.5–250 ?g.mL-1for pseudoephdrine HCI and pheniramine maleate, 1–250 ?g.mL-1for guaifenisin, 2.5-250 ?g.mL-1for chlorpheniramine maleate and triprolidine HCI, 5-250 ?g.mL-1for pyrilamine maleate and diphenhydramine HCI, 10-20 ?g.mL-1for dextromethorphan HBr with correlation coefficients better than 0.9993. The relative standard deviations of the intraday and interday were all less than 4%. Conclusion: The proposed liquid chromatographic method was successfully applied for the routine analysis of these compounds in different cough and cold pharmaceutical preparations such as syrups and tablets. © 2014, International Journal of Pharmacy and Pharmaceutical Science. All rights reserved.en_US
dc.identifier.endpage428en_US
dc.identifier.issn0975-1491
dc.identifier.issue10en_US
dc.identifier.scopus2-s2.0-84908348101en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage421en_US
dc.identifier.urihttps://hdl.handle.net/11616/90790
dc.identifier.volume6en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherInternational Journal of Pharmacy and Pharmaceutical Scienceen_US
dc.relation.ispartofInternational Journal of Pharmacy and Pharmaceutical Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectActive ingredients cough and cold pharmaceuticalsen_US
dc.subjectHigh performance liquid chromatographyen_US
dc.subjectValidationen_US
dc.titleHPLC method development and validation: Simultaneous determination of active ingredients in cough and cold pharmaceuticalsen_US
dc.typeArticleen_US

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