Phenethyl isothiocyanate Regulates the Cancer Stem Cell Phenotype of SNU449 Hepatocellular Carcinoma Cells via STAT3-CD44 Axis

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dc.authorscopusid36698899000
dc.contributor.authorSatilmiş B.
dc.date.accessioned2024-08-04T20:01:01Z
dc.date.available2024-08-04T20:01:01Z
dc.date.issued2023
dc.departmentİnönü Üniversitesien_US
dc.description.abstractCancer stem cells play an essential role in resistance to therapy, invasion, metastasis, and recurrence. CD44 is one of the well-known surface markers for hepatocellular carcinoma, and its expression level is related to poor survival and a high recurrence rate. The effect of phenethyl isothiocyanate (PEITC) on SNU449 hepatocellular carcinoma cell line cancer stem cells is not known. The goal of the present study was to investigate whether PEITC regulates the cancer stem cell phenotype of SNU449 cells. Cell viability, colony formation, and wound healing assays were performed to determine proliferative and migratory characteristics. Caspase 3, CD44, Akt/ mTOR, and p38/STAT3 protein expression levels were measured by Western blotting. Compared to control confluence, gap fill, and migration rate were increased while half gap time was decreased in PEITC-treated cells. Compared to control-treated cells, CD44 (3.2-fold) and p-STAT3 (2.44-fold) protein expressions were upregulated in PEITC-treated cells. Results of this study suggest that STAT3-mediated upregulation of CD44 leads to the gain of cancer stem cell phenotype of PEITC-treated SNU449 cells. © 2023 Society of Pharmaceutical Sciences of Ankara (FABAD). All rights reserved.en_US
dc.identifier.doi10.55262/fabadeczacilik.1356769
dc.identifier.endpage560en_US
dc.identifier.issn1300-4182
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-85174928080en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage549en_US
dc.identifier.trdizinid1209758en_US
dc.identifier.urihttps://doi.org/10.55262/fabadeczacilik.1356769
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/1209758
dc.identifier.urihttps://hdl.handle.net/11616/91193
dc.identifier.volume48en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.publisherSociety of Pharmaceutical Sciences of Ankara (FABAD)en_US
dc.relation.ispartofFabad Journal of Pharmaceutical Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectcancer stem cellen_US
dc.subjecte-mail:basri.satilmis@inonu.edu.tren_US
dc.subjecthepatocellular carcinomaen_US
dc.subjectPhenethyl isothiocyanateen_US
dc.titlePhenethyl isothiocyanate Regulates the Cancer Stem Cell Phenotype of SNU449 Hepatocellular Carcinoma Cells via STAT3-CD44 Axisen_US
dc.typeArticleen_US

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