The Nanosuspension Formulations of Daidzein: Preparation and In Vitro Characterization

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dc.authoridugur kaplan, afife busra/0000-0003-2222-8789
dc.authoridOzturk, Naile/0000-0002-7617-8433
dc.authorwosidugur kaplan, afife busra/AAY-2218-2021
dc.authorwosidÇetin, Meltem/ABI-4879-2020
dc.authorwosidOznuluer, Tuba/AAD-4079-2020
dc.authorwosidOzturk, Naile/F-3650-2017
dc.contributor.authorUgur Kaplan, Afife Busra
dc.contributor.authorOzturk, Naile
dc.contributor.authorCetin, Meltem
dc.contributor.authorVural, Imran
dc.contributor.authorOznuluer Ozer, Tuba
dc.date.accessioned2024-08-04T20:10:09Z
dc.date.available2024-08-04T20:10:09Z
dc.date.issued2022
dc.departmentİnönü Üniversitesien_US
dc.description.abstractObjectives: Daidzein (DZ), a water-insoluble isoflavone, has many beneficial effects (anti-inflammatory, antioxidant, and anticancer effects, etc.) on human health. DZ has a very low oral bioavailability related to its physicochemical properties (low solubility, intense metabolism of DZ in the intestine and liver). This study aimed to prepare and in vitro characterize the nanosuspension formulations of DZ to improve the poor solubility and efficacy of DZ. Materials and Methods: DZ nanosuspension formulations were prepared with media milling technique using zirconium oxide beads as milling media. Pluronic F127 and polyvinylpyrrolidone (PVP) K30 (formulation A; F-A) and sodium dodecyl sulfate (SDS) (SDS + pluronic F127 + PVP K30; formulation B; F-B) were used as stabilizers. The nanosuspension formulations were evaluated for morphological properties, particle sizes, zeta potential, DZ content, saturation solubility, dissolution, and their cytotoxic effects on RG2 glioblastoma tumor cells. Results: F-A and F-B formulations were nanosized (in the range of about 181-235 nm) and had negative zeta potential values before and after lyophilization. The DZ content of F-A and F-B formulations were found to be 93.68 +/- 0.78% and 89.75 +/- 0.49%, respectively. Fourier transform infrared spectroscopy analysis showed that there was no significant interaction between DZ and the excipients. Differential scanning calorimetry and X-ray diffraction analyses confirmed no change in the crystal structure of DZ in F-A and F-B formulations. Conclusion: In this study, the nanosuspension formulations were successfully prepared and characterized in vitro. Nanosuspension formulations increased the saturation solubility, dissolution rate, and cytotoxic effect of DZ.en_US
dc.identifier.doi10.4274/tjps.galenos.2021.81905
dc.identifier.endpage92en_US
dc.identifier.issn1304-530X
dc.identifier.issn2148-6247
dc.identifier.issue1en_US
dc.identifier.pmid35227054en_US
dc.identifier.scopus2-s2.0-85122739192en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage84en_US
dc.identifier.trdizinid525166en_US
dc.identifier.urihttps://doi.org/10.4274/tjps.galenos.2021.81905
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/525166
dc.identifier.urihttps://hdl.handle.net/11616/92621
dc.identifier.volume19en_US
dc.identifier.wosWOS:000767290000011en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherGalenos Publ Houseen_US
dc.relation.ispartofTurkish Journal of Pharmaceutical Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCytotoxicityen_US
dc.subjectdaidzeinen_US
dc.subjectFTIR analysisen_US
dc.subjectnanosuspensionen_US
dc.subjectmedia millingen_US
dc.titleThe Nanosuspension Formulations of Daidzein: Preparation and In Vitro Characterizationen_US
dc.typeArticleen_US

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