Production of methionine ?- lyase in recombinant Citrobacter freundii bearing the hemoglobin gene
Küçük Resim Yok
Tarih
2011
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Korean Society Biochemistry & Molecular Biology
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
The production of antileukemic enzyme methionine gamma-lyase (MGL) in distinctly related bacteria, Citrobacter freundii and in their recombinants expressing the Vitresocilla hemoglobin (VHb) has been studied. This study concerns the potential of Citrobacter freundii expressing the Vitreoscilla hemoglobin gene (vgb) for the methionine gamma- liyase production. Methionine gamma- liyase production by Citrobacter freundii and its vgb(-) and vgb(+) bearing recombinant strain was studied in shake-flasks under 200 rpm agitation, culture medium and 30 degrees C in a time-course manner. The vgb(+) and especially the carbon type had a dramatic effect on methionine gamma- liyase production. The vgb(+) strain of C. freundii had about 2-fold and 3.1-fold higher levels of MGL than the host and vgb(-) strain, respectively. [BMB reports 2011; 44(9): 590-594]
Açıklama
Anahtar Kelimeler
Antitumour efficacy, Citrobacter freundii, Methionine gamma-lyase, Vitreoscilla hemoglobin
Kaynak
Bmb Reports
WoS Q Değeri
Q4
Scopus Q Değeri
N/A
Cilt
44
Sayı
9
