Determining expression of miRNAs that potentially regulate STAT5A and 5B in dasatinib-sensitive K562 cells

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dc.authoridTezcanli Kaymaz, Burcin/0000-0003-1832-1454
dc.authoridSahin, Fahri/0000-0001-9315-8891
dc.authoridKosova, Buket/0000-0003-3636-6082
dc.authoridComert, Melda/0000-0002-7798-4349
dc.authorwosidSaydam, Guray/W-3827-2017
dc.authorwosidTezcanli Kaymaz, Burcin/A-1225-2018
dc.authorwosidSahin, Fahri/AAA-2768-2020
dc.authorwosidSoyer, Nur/JYO-9281-2024
dc.authorwosidKosova, Buket/AAG-4736-2019
dc.authorwosidComert, Melda/ABH-5764-2020
dc.contributor.authorYilmaz, Asu Fergun
dc.contributor.authorKaymaz, Burcin
dc.contributor.authorAktan, Cagdas
dc.contributor.authorSoyer, Nur
dc.contributor.authorKosova, Buket
dc.contributor.authorGunes, Ajda
dc.contributor.authorSahin, Fahri
dc.date.accessioned2024-08-04T20:09:52Z
dc.date.available2024-08-04T20:09:52Z
dc.date.issued2017
dc.departmentİnönü Üniversitesien_US
dc.description.abstractIn the era of tyrosine kinase inhibitors, resistance still constitutes a problem in chronic myeloid leukemia (CML) patients; thus, new pathway-specific inhibitors like miRNAs have become important in the treatment of refractory patients. There are no satisfying data regarding the miRNAs and anti-miRNA treatment targeting STAT5A and 5B. In this study, we first researched the effect of dasatinib on apoptosis in the CML cell line K562. The expressions of miRNAs possibly targeting both STAT5A and 5B were then determined. The down-and upregulation of the miRNAs were compared using the Delta Delta CT method. At the last stage of the study, we used a new primer probe in order to validate the results. The level of hsa-miR-940 was decreased 4.4 times and the levels of hsa-miR-527 and hsa-miR-518a-5p were increased 12.1 and 8 times, respectively, in the dasatinib-treated group when compared to the control group. We detected similar results in the validation step. As a conclusion, we determined the expression profiles of miRNAs targeting STAT5A and 5B that had an important role in the pathogenesis of CML. The data obtained could lead to determining new therapeutic targets for CML patients.en_US
dc.description.sponsorshipScientific Research Projects Branch Directorate of Ege University, Turkey [2013 - TIP-019]; Hematology Specialist Associationen_US
dc.description.sponsorshipThis study was financially supported by the Scientific Research Projects Branch Directorate of Ege University, Turkey (2013 - TIP-019) and the Hematology Specialist Association.en_US
dc.identifier.doi10.3906/biy-1705-66
dc.identifier.endpage934en_US
dc.identifier.issn1300-0152
dc.identifier.issn1303-6092
dc.identifier.issue6en_US
dc.identifier.pmid30814857en_US
dc.identifier.scopus2-s2.0-85038444700en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage926en_US
dc.identifier.trdizinid255579en_US
dc.identifier.urihttps://doi.org/10.3906/biy-1705-66
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/255579
dc.identifier.urihttps://hdl.handle.net/11616/92487
dc.identifier.volume41en_US
dc.identifier.wosWOS:000418253100008en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTubitak Scientific & Technological Research Council Turkeyen_US
dc.relation.ispartofTurkish Journal of Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectmiRNA expressionsen_US
dc.subjectSTAT5Aen_US
dc.subjectSTAT5Ben_US
dc.subjectchronic myeloid leukemiaen_US
dc.subjectdasatiniben_US
dc.subjectK562 cellsen_US
dc.titleDetermining expression of miRNAs that potentially regulate STAT5A and 5B in dasatinib-sensitive K562 cellsen_US
dc.typeArticleen_US

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