The inhibition of Src kinase suppresses the production of matrix metalloproteinases in from synovial fibroblasts and inhibits MAPK and STATs pathways

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dc.contributor.authorYalçın Kehribar, Demet
dc.contributor.authorÖzgen, Metin
dc.contributor.authorYolbaş, Servet
dc.contributor.authorYıldırım, Ahmet
dc.contributor.authorTürkmen, Neşe Başak
dc.contributor.authorOnalan, Ebru
dc.contributor.authorÇiftci, Osman
dc.contributor.authorÖzercan, İbrahim Hanifi
dc.contributor.authorKoca, Süleyman S.
dc.date.accessioned2022-02-08T07:59:40Z
dc.date.available2022-02-08T07:59:40Z
dc.date.issued2021
dc.departmentİnönü Üniversitesien_US
dc.description.abstractAbstract: Background/aim: The purpose of this study was to investigate the antiarthritic potentials of the inhibition of Src kinase in vivo and in vitro settings. Materials and methods: Arthritis was induced by intradermal injection of chicken type II collagen combined with incomplete Freund’s adjuvant (collagen induced arthritis [CIA] model) in Wistar albino rats. One day after the onset of arthritis, dasatinib, a potent Src kinase inhibitor, (5 mg/kg/day) was given via oral gavage. Tissue Src, Fyn, MAPK and STAT mRNA expressions were determined by real-time polymerase chain reaction. On the other hand, fibroblast like synoviocytes (FLSs) were harvested patients with rheumatoid arthritis (RA) undergoing surgical knee joint replacement. FLSs were stimulated with cytokines and dasatinib was added in different concentrations. MMP –1, –3, and –13 levels in FLSs culture were determined by ELISA. Results: The tissue mRNA expressions of Src, Fyn, MAPK and STATs were increased in the arthritis CIA group compared to the control group. Their mRNA expressions in the CIA + dasatinib group were decreased and similar in the control group. In in vitro setting, MMP –1, –3, and –13 expressions from FLSs induced by IL-1? and TNF-? were increased, while dasatinib suppressed their productions from FLSs. Conclusion: The present study shows that the inhibition of Src kinase has antiarthritic potentials in both in vivo and in vitro settings. Src kinase inhibition may be candidate to further research in human RA. Key words: Rheumatoid arthritis, collagen induced arthritis, src kinase, matrix metalloproteinaseen_US
dc.identifier.citationKEHRİBAR D. Y,ÖZGEN M,YOLBAŞ S,YILDIRIM A,TÜRKMEN N. B,ONALAN E,ÇİFTÇİ O,ÖZERCAN İ. H,KOCA S. S (2021). The inhibition of Src kinase suppresses the production of matrix metalloproteinases in from synovial fibroblasts and inhibits MAPK and STATs pathways. Turkish Journal of Medical Sciences, 51(4), 2142 - 2149. Doi: 10.3906/sag-2008-274en_US
dc.identifier.doi10.3906/sag-2008-274en_US
dc.identifier.endpage2149en_US
dc.identifier.issn1300-0144
dc.identifier.issn1303-6165
dc.identifier.issue4en_US
dc.identifier.pmid33714238en_US
dc.identifier.scopus2-s2.0-85106721365en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage2142en_US
dc.identifier.trdizinid481010en_US
dc.identifier.urihttps://doi.org/10.3906/sag-2008-274
dc.identifier.urihttps://hdl.handle.net/11616/46802
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/481010
dc.identifier.volume51en_US
dc.identifier.wosWOS:000691664800007en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakTR-Dizinen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.relation.ispartofTurkish Journal of Medical Sciencesen_US
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.titleThe inhibition of Src kinase suppresses the production of matrix metalloproteinases in from synovial fibroblasts and inhibits MAPK and STATs pathwaysen_US
dc.typeArticleen_US

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