Determination of the Nucleic Acid Constituents in Edible Mushrooms by High-Performance Liquid Chromatography with Diode Array Detection (HPLC-DAD)
Küçük Resim Yok
Tarih
2024
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Taylor & Francis Inc
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Nucleosides and nucleobases in edible mushrooms collected from the Tekirdag region of Turkey were determined by high performance liquid chromatography (HPLC). Separation of compounds was accomplished by gradient elution on a C18 column. A mixture of methanol (MeOH):50 mM sodium phosphate buffer (1:99, v/v) at pH 3.70 and methanol:50 mM sodium phosphate buffer (20:80) at pH 6.20 was used as the solvent. Two methods were used to treat the lyophilized mushrooms: ultrasound-assisted solid-liquid extraction (US-SLE) and pressurized (accelerated) solid-liquid extraction (PSLE). Deionized water was used as the extraction solvent. The extraction temperature and time were optimized. Fourteen nucleosides and nucleobases were simultaneously monitored at 254 nm within 25 min. Ultrasound-assisted solid-liquid was demonstrated to be more effective, perhaps because the pressurized liquid extraction system is a closed system and the matrix-solvent interaction may be insufficient. Adenosine, guanosine and uridine were the most abundant compounds in all studied mushrooms.
Açıklama
Anahtar Kelimeler
Edible mushrooms, high-performance liquid chromatography with diode array detection (HPLC-DAD), nucleobases, nucleosides, ultrasound-assisted solid-liquid extraction
Kaynak
Analytical Letters
WoS Q Değeri
N/A
Scopus Q Değeri
Q3
