Determination of the Nucleic Acid Constituents in Edible Mushrooms by High-Performance Liquid Chromatography with Diode Array Detection (HPLC-DAD)

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dc.contributor.authorCamci, Ibrahim
dc.contributor.authorBuyuktuncel, S. Ebru
dc.date.accessioned2024-08-04T20:56:12Z
dc.date.available2024-08-04T20:56:12Z
dc.date.issued2024
dc.departmentİnönü Üniversitesien_US
dc.description.abstractNucleosides and nucleobases in edible mushrooms collected from the Tekirdag region of Turkey were determined by high performance liquid chromatography (HPLC). Separation of compounds was accomplished by gradient elution on a C18 column. A mixture of methanol (MeOH):50 mM sodium phosphate buffer (1:99, v/v) at pH 3.70 and methanol:50 mM sodium phosphate buffer (20:80) at pH 6.20 was used as the solvent. Two methods were used to treat the lyophilized mushrooms: ultrasound-assisted solid-liquid extraction (US-SLE) and pressurized (accelerated) solid-liquid extraction (PSLE). Deionized water was used as the extraction solvent. The extraction temperature and time were optimized. Fourteen nucleosides and nucleobases were simultaneously monitored at 254 nm within 25 min. Ultrasound-assisted solid-liquid was demonstrated to be more effective, perhaps because the pressurized liquid extraction system is a closed system and the matrix-solvent interaction may be insufficient. Adenosine, guanosine and uridine were the most abundant compounds in all studied mushrooms.en_US
dc.description.sponsorshipInonu University Research Council [2019/1957]en_US
dc.description.sponsorshipThis research was supported by the Inonu University Research Council (Project no: 2019/1957).en_US
dc.identifier.doi10.1080/00032719.2024.2375387
dc.identifier.issn0003-2719
dc.identifier.issn1532-236X
dc.identifier.scopus2-s2.0-85197797530en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.urihttps://doi.org/10.1080/00032719.2024.2375387
dc.identifier.urihttps://hdl.handle.net/11616/102104
dc.identifier.wosWOS:001268421400001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Incen_US
dc.relation.ispartofAnalytical Lettersen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectEdible mushroomsen_US
dc.subjecthigh-performance liquid chromatography with diode array detection (HPLC-DAD)en_US
dc.subjectnucleobasesen_US
dc.subjectnucleosidesen_US
dc.subjectultrasound-assisted solid-liquid extractionen_US
dc.titleDetermination of the Nucleic Acid Constituents in Edible Mushrooms by High-Performance Liquid Chromatography with Diode Array Detection (HPLC-DAD)en_US
dc.typeArticleen_US

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