Alkolün Endokrin Pankreas Üzerine Histolojik Etkileri+
Yükleniyor...
Dosyalar
Tarih
2002
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
İnönü Üniversitesi Tıp Fakültesi Dergisi
Erişim Hakkı
Attribution 3.0 United States
info:eu-repo/semantics/openAccess
info:eu-repo/semantics/openAccess
Özet
Bu çalışmada; uzun süreli alkol alımının, sıçanların pankreas Langerhans adacıkları üzerine olası histolojik etkilerinin araştırılması amaçlandı. Materyal ve Metod: Çalışmada 17 adet Wistar albino sıçan kullanıldı. Laboratuvar hayvanları deney ve kontrol olmak üzere iki gruba ayrıldı. Deney grubu 6 ay boyunca %7.2 alkol içeren modifiye sıvı diyet (MSD) ile beslendiler. Kontrol sıçanları da izokalorik olarak etanolsüz MSD ile beslendi. Deney süresinin sonunda sıçanlar servikal dislokasyonla öldürülüp, pankreasları alındı. Tesbit işleminden sonra, dokulara rutin histolojik prosedür uygulandı ve parafin içine gömüldü. Parafin bloklardan 6 ?m kalınlığında kesitler alınıp; H-E, Crosmann trikrom, toluidin blue, Grimelius gümüşleme ve aldehit fuksin boyama metodları ile boyandı ve ışık mikroskopta incelendi. Bulgular: Kontrol grubu ile karşılaştırıldığında alkol grubunda Langerhans adası içinde kanal oluşumları ve genişlemiş kapiller damarlar izlendi. Langerhans adasını oluşturan hücrelerde ve intersellüler alanda değişik büyüklükte vakuoller saptandı. Alfa hücreleri kontrol ve alkol grubunda adacığın periferinde, yüzük şeklinde bir dağılım göstermekteydi. Ancak alkol grubunda alfa hücre granülleri daha yoğun olarak gözlendi. Alkol grubundaki beta hücreleri adacığın merkezinde ve azalan granülleri ile dikkat çekti. Sonuç: Alkolün doğrudan ya da dolaylı olarak endokrin pankreas üzerinde yapısal bozukluklara neden olduğu sonucuna varıldı.
The purpose of this study was to investigate the possible effects of chronic ethanol intake on the endocrine pancreas of rats. Material and Metods: The study was performed on 17 male Wistar albino rats. The laboratory animals divided into two groups as experiment and control. The experimental group was fed with a modified liquid diet (MLD) containing 7.2 % of ethanol during six months. Control rats were fed by isocaloric MLD without ethanol. At the end of the experiment rats were sacrified by cervical dislocation and their pancreas tissue were excised. After routine tissue process, pancreas were embedded in paraffin. 6 ? section were stained with H-E, Crossman's tricrome, toluidin blue, Grimelius and aldehyde fuchsin methods and examined by light microscope. Results: When compared with control group ethanol group showed ducts and widened capillaries in islets of Langerhans. Vacuolization were detected in islets of Langerhans in both intracellular and extracellular zones. Alpha cells presented ring like distribution at the periphery of islets of Langerhans in both examined groups of rats, but ethanol groups showed increased density of granules. In contrast in ethanol group, Beta cells were localized in the center of the islets and showed decreased density of granules. Conclusion: We concluded that ethanol has structural damaging effects both directly and indirectly on endocrine pancreas directly or indirectly.
The purpose of this study was to investigate the possible effects of chronic ethanol intake on the endocrine pancreas of rats. Material and Metods: The study was performed on 17 male Wistar albino rats. The laboratory animals divided into two groups as experiment and control. The experimental group was fed with a modified liquid diet (MLD) containing 7.2 % of ethanol during six months. Control rats were fed by isocaloric MLD without ethanol. At the end of the experiment rats were sacrified by cervical dislocation and their pancreas tissue were excised. After routine tissue process, pancreas were embedded in paraffin. 6 ? section were stained with H-E, Crossman's tricrome, toluidin blue, Grimelius and aldehyde fuchsin methods and examined by light microscope. Results: When compared with control group ethanol group showed ducts and widened capillaries in islets of Langerhans. Vacuolization were detected in islets of Langerhans in both intracellular and extracellular zones. Alpha cells presented ring like distribution at the periphery of islets of Langerhans in both examined groups of rats, but ethanol groups showed increased density of granules. In contrast in ethanol group, Beta cells were localized in the center of the islets and showed decreased density of granules. Conclusion: We concluded that ethanol has structural damaging effects both directly and indirectly on endocrine pancreas directly or indirectly.
Açıklama
İnönü Üniversitesi Tıp Fakültesi Dergisi
9(3) 163-168 (2002)
Anahtar Kelimeler
Alkol, Langerhans Adacığı, Sıçan, Mikroskop, Alcohol, Langerhans Islets, Rat, Microscope
Kaynak
İnönü Üniversitesi Tıp Fakültesi Dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
9
Sayı
3
Künye
Vardı, Nigar ;Otlu, Ali ;Uçar, Muharrem ;Öztürk, Feral ;İnönü Üniversitesi Tıp Fakültesi Dergisi 9(3) 163-168 (2002)
