Antiprotozoal Activity of Artemisia vulgaris and Berberis vulgaris Against Leishmania major and Trichomonas vaginalis
Küçük Resim Yok
Tarih
2026
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Springer Int Publ Ag
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Purpose Leishmania major and Trichomonas vaginalis infections pose a significant global health burden, while current treatments are limited by toxicity, resistance, and restricted accessibility. This study aimed to evaluate the in vitro and ex vivo antileishmanial effects of Artemisia vulgaris and Berberis vulgaris extracts against L. major, as well as their in vitro antitrichomonal activity against T. vaginalis trophozoites. Methods Ethanolic extracts of A. vulgaris and B. vulgaris were tested against L. major promastigotes, intracellular amastigotes, and T. vaginalis trophozoites. Parasite viability was determined by CellTiter-Glo (R), microscopy, and rescue-transformation assays, and selectivity indices (SI) were calculated. Amphotericin B and metronidazole served as reference drugs. Results Both extracts exhibited low cytotoxicity in THP-1 macrophages (A. vulgaris CC50 = 465.2 & micro;g/mL; B. vulgaris = 357.7 & micro;g/mL). Against L. major, B. vulgaris showed greater potency (IC50 = 76.8 & micro;g/mL; SI = 4.7 for amastigotes) than A. vulgaris (IC50 = 179.7 & micro;g/mL; SI = 2.6). Both extracts reduced intracellular parasite burden in a dose-dependent manner, achieving complete clearance at non-cytotoxic concentrations (>= 300 & micro;g/mL). In T. vaginalis, the extracts induced concentration-dependent inhibition, with IC50 values of 68.9 & micro;g/mL (B. vulgaris, SI = 5.2) and 104.4 & micro;g/mL (A. vulgaris, SI = 4.5). Conclusion Both extracts exhibited selective, dose-dependent antiprotozoal activity, with B. vulgaris showing superior efficacy, particularly against intracellular L. major and T. vaginalis. These results highlight their potential as natural antiprotozoal sources, warranting further studies on active constituents, mechanisms, and in vivo efficacy.
Açıklama
Anahtar Kelimeler
Antileishmanial activity, Antitrichomonal activity, Artemisia vulgaris, Berberis vulgaris, Leishmania major, Trichomonas vaginalis
Kaynak
Acta Parasitologica
WoS Q Değeri
Q2
Scopus Q Değeri
Q3
Cilt
71
Sayı
1
