Antiprotozoal Activity of Artemisia vulgaris and Berberis vulgaris Against Leishmania major and Trichomonas vaginalis

Basit Öğe Kaydı
dc.contributor.authorAksoy, Tulay
dc.contributor.authorGirginkardesler, Nogay
dc.contributor.authorBalcioglu, Ibrahim Cuneyt
dc.contributor.authorKilimcioglu, Ali Ahmet
dc.date.accessioned2026-04-04T13:37:26Z
dc.date.available2026-04-04T13:37:26Z
dc.date.issued2026
dc.departmentİnönü Üniversitesi
dc.description.abstractPurpose Leishmania major and Trichomonas vaginalis infections pose a significant global health burden, while current treatments are limited by toxicity, resistance, and restricted accessibility. This study aimed to evaluate the in vitro and ex vivo antileishmanial effects of Artemisia vulgaris and Berberis vulgaris extracts against L. major, as well as their in vitro antitrichomonal activity against T. vaginalis trophozoites. Methods Ethanolic extracts of A. vulgaris and B. vulgaris were tested against L. major promastigotes, intracellular amastigotes, and T. vaginalis trophozoites. Parasite viability was determined by CellTiter-Glo (R), microscopy, and rescue-transformation assays, and selectivity indices (SI) were calculated. Amphotericin B and metronidazole served as reference drugs. Results Both extracts exhibited low cytotoxicity in THP-1 macrophages (A. vulgaris CC50 = 465.2 & micro;g/mL; B. vulgaris = 357.7 & micro;g/mL). Against L. major, B. vulgaris showed greater potency (IC50 = 76.8 & micro;g/mL; SI = 4.7 for amastigotes) than A. vulgaris (IC50 = 179.7 & micro;g/mL; SI = 2.6). Both extracts reduced intracellular parasite burden in a dose-dependent manner, achieving complete clearance at non-cytotoxic concentrations (>= 300 & micro;g/mL). In T. vaginalis, the extracts induced concentration-dependent inhibition, with IC50 values of 68.9 & micro;g/mL (B. vulgaris, SI = 5.2) and 104.4 & micro;g/mL (A. vulgaris, SI = 4.5). Conclusion Both extracts exhibited selective, dose-dependent antiprotozoal activity, with B. vulgaris showing superior efficacy, particularly against intracellular L. major and T. vaginalis. These results highlight their potential as natural antiprotozoal sources, warranting further studies on active constituents, mechanisms, and in vivo efficacy.
dc.description.sponsorshipInonu University
dc.description.sponsorshipOpen access funding provided by the Scientific and Technological Research Council of Turkiye (TUB & Idot;TAK).
dc.identifier.doi10.1007/s11686-026-01224-1
dc.identifier.issn1230-2821
dc.identifier.issn1896-1851
dc.identifier.issue1
dc.identifier.orcid0000-0002-9680-4294
dc.identifier.orcid0000-0003-3397-8411
dc.identifier.pmid41697430
dc.identifier.scopus2-s2.0-105030145190
dc.identifier.scopusqualityQ3
dc.identifier.urihttps://doi.org/10.1007/s11686-026-01224-1
dc.identifier.urihttps://hdl.handle.net/11616/109805
dc.identifier.volume71
dc.identifier.wosWOS:001693238900001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer Int Publ Ag
dc.relation.ispartofActa Parasitologica
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WOS_20250329
dc.subjectAntileishmanial activity
dc.subjectAntitrichomonal activity
dc.subjectArtemisia vulgaris
dc.subjectBerberis vulgaris
dc.subjectLeishmania major
dc.subjectTrichomonas vaginalis
dc.titleAntiprotozoal Activity of Artemisia vulgaris and Berberis vulgaris Against Leishmania major and Trichomonas vaginalis
dc.typeArticle

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu