Ameliorative effects of aminoguanidine on rat aorta in Streptozotocin-induced diabetes and evaluation of ?-SMA expression

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dc.authoridVardı, Nigar/0000-0003-0576-1696
dc.authoridTaslidere, Elif/0000-0003-1723-2556
dc.authorwosidElbe, Hulya/D-5261-2015
dc.authorwosidVardı, Nigar/C-9549-2018
dc.authorwosidTaslidere, Elif/ABI-8046-2020
dc.contributor.authorElbe, Hulya
dc.contributor.authorVardi, Nigar
dc.contributor.authorOrman, Dogan
dc.contributor.authorTaslidere, Elif
dc.contributor.authorYildiz, Azibe
dc.date.accessioned2024-08-04T20:09:47Z
dc.date.available2024-08-04T20:09:47Z
dc.date.issued2014
dc.departmentİnönü Üniversitesien_US
dc.description.abstractObjective: Diabetes mellitus is one of the chronic metabolic diseases which is characterized by microvascular and macrovascular complications. This study was designed to investigate the improving the effects of amnioguanidine on aortic damage in a streptozotocin (STZ) induced diabetic rat model. Methods: Thirty-two male Sprague-Dawley rats divided into four groups as follows: Control, Aminoguanidine, Diabetes, and Diabetes+Aminoguanidine. Experimental diabetes was induced by single dose STZ (45 mg/kg) intraperitoneally. After administration of STZ, the DM+AMG group began to receive AMG (1 g/L) was prepared by dissolving in tap water during 10 weeks. At the end of the study, blood glucose levels were determined and rats were sacrified by ketamine anesthesia. Following routine tissue process, aortas were embedded in paraffin. Histochemical (H-E and Orcein) and immunohistochemical alpha-smooth muscle actin (alpha-SMA) stains were applied and the sections examined by light microscope. Statistical analysis was carried out using the SPSS 13.0 statistical program. Results: The rats in diabetes group had significantly higher blood glucose levels than the rats of control. The main histological alterations were detected in tunica media such as extensive thickening (414.32 +/- 9.62 mu m), irregular of elastic fibers and intensive alpha-SMA staining in diabetic rats. The thickness of tunica media was statistically increased in DM group, when compared with the control group (p<0.001). On the other hand, AMG prevented disorganization of elastic fibers and overexpression of alpha-SMA. The mean thickness of tunica media was decreased significantly in DM+AMG (319.16 +/- 6.53 mu m) compared with the DM group (p<0.001). Conclusion: Our results demonstrate that AMG treatment may protect the impairment of aort structure at histological level.en_US
dc.description.sponsorshipInonu University [2010/70]en_US
dc.description.sponsorshipThis study was financially supported by a grant from Scientific Research Fund of Inonu University (Project number: 2010/70). The our gratitude also extends to the Department of Histology and Embryology, Faculty of Medicine for their and support. We would like to thanks to Prof. Dr. Saim Yologlu for him statistical evaluation.en_US
dc.identifier.doi10.5152/akd.2014.5047
dc.identifier.endpage684en_US
dc.identifier.issn2149-2263
dc.identifier.issn2149-2271
dc.identifier.issue8en_US
dc.identifier.pmid25188757en_US
dc.identifier.scopus2-s2.0-84919819085en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage679en_US
dc.identifier.trdizinid235457en_US
dc.identifier.urihttps://doi.org/10.5152/akd.2014.5047
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/235457
dc.identifier.urihttps://hdl.handle.net/11616/92426
dc.identifier.volume14en_US
dc.identifier.wosWOS:000348352900004en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTurkish Soc Cardiologyen_US
dc.relation.ispartofAnatolian Journal of Cardiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectdiabetesen_US
dc.subjectaminoguanidineen_US
dc.subjectalpha-smooth muscle actinen_US
dc.subjectnitric oxideen_US
dc.subjectaortaen_US
dc.subjectraten_US
dc.titleAmeliorative effects of aminoguanidine on rat aorta in Streptozotocin-induced diabetes and evaluation of ?-SMA expressionen_US
dc.typeArticleen_US

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