Antibacterial effectiveness of alkalinized ropivacaine and bupivacaine
Küçük Resim Yok
Tarih
2007
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Aim: We aimed to investigate the antibacterial effects of alkalinized ropivacaine and bupivacaine on the Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa Materials and Methods: In this study, solutions of bupivacaine 0.5%, ropivaeaine 0.2%, bupivakain 0.5%+NaHCO3, ropivakain 0.2%+NaHCO3 with bactorial cultures' E. coli, S. aureus and P. aeruginosa were used. Bacteria were grown on standard blood agar. Bacterial cultures were prepared in a density of 0.5 McFarlands units (10 8 efu ml-1) with sterile saline 0.9% and each bacterial solution was further diluted in Mueller-Hinton broth to obtain standard inocula (105 cfu ml-1). The tested solutions (2 ml) and controls (2 ml, physiological saline) were added to 2 ml of standard bacterial preparations. Aliquats (3 ml) were vortexed and pipetted into sterile polystyrene spectrophotometer cuvets and it was incubated at 37°C. The optical density at 540 nm was measured at 0, 3 and 6 h by spectrophotometer. Results: While hupivacaine inhibited the growth of S. aureus and E. coli at all measurements times, P. aeruginosa inhibited at only 6 h (p<0.05). Alkalinized bupivacaine inhibited the growth of S. aureus at 0 h and E. coli at 0 and 6 h (p<0.05). Antibacterial effects of hupivacaine on E. coli was significantly higher than alkalinized bupivacaine at 6h (p<0.05). While ropivaeaine more inhibited the growth of S. aureus at 0 and 3 h than alkalinized ropivauaine, alkalinized ropivacaine less inhibited it according to the control (p<0.05). While both ropivacainc and alkalinized ropivakain inhibited the growth of E. coli at 3 and 6 h, at 0 h only ropivacaine inhibited it (p<0.05). Ropivacaine and alkalinized ropivaeaine inhibited the growth of P. aeruginosa at 3 h (p<0.05). Conclusion: It has been determined that bupivacaine has antibacterial effect and alkalinization of bupivacaine does not change it, ropivacaine has poor antibacterial effect and it more reduced with alkalinization of ropivacaine.
Açıklama
Anahtar Kelimeler
Bupivacaine, Microbiological phenomena, Ropivacaine, Sodium bicarbonate
Kaynak
Turk Anesteziyoloji ve Reanimasyon Dernegi Dergisi
WoS Q Değeri
Scopus Q Değeri
N/A
Cilt
35
Sayı
1
