Natural Vanillin Production from Isoeugenol by Using Pseudomonas putida in Biphasic Bioconversion Medium

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dc.authoridYilmaztekin, Murat/0000-0002-5667-9169
dc.authoridKarakaya, Huseyin/0000-0002-6311-473X
dc.authorwosidYilmaztekin, Murat/N-9692-2013
dc.authorwosidKarakaya, Huseyin/ABI-7701-2020
dc.contributor.authorKarakaya, Huseyin
dc.contributor.authorYilmaztekin, Murat
dc.date.accessioned2024-08-04T20:10:14Z
dc.date.available2024-08-04T20:10:14Z
dc.date.issued2022
dc.departmentİnönü Üniversitesien_US
dc.description.abstractVanillin, is one of the most demanded flavoring agents in the world. Because of insufficient supply of natural vanillin, market demand is usually supplied by synthetic ones. In this study, it was investigated possibility of usage biphasic system in bioconversion of isoeugenol to vanillin by Pseudomonas putida (HUT 8100). Organic phase was composed of isoeugenol while biocatalyst, P. putida culture, was dispersed in aqueous phosphate solution. Isoeugenol was used as sole carbon source in concentrations ranging between 50-600 g L-1. Incubation was performed at 28 degrees C, at pH 6.3 and 180 rpm shaking. Effect of initial substrate concentration and bioconversion time were investigated. Isoeugenol and vanillin amounts in medium were simultaneously analyzed in HPLC system. After 120 h incubation, vanillin reached the its highest level when 400 g L-1 isoeugenol was applied in medium. In specified conditions, it was achieved to produce 11.95 g L-1 vanillin with 6.2% molar yield within 15 days of bioconversion. It is thought that, obtained result by using biphasic system is very important for the industrial applications in production of natural vanillin via bioconversion.en_US
dc.description.sponsorshipInonu University Scientific Research Projects Coordination Unit [2015/17]en_US
dc.description.sponsorshipThis study was supported by Inonu University Scientific Research Projects Coordination Unit (Project number: 2015/17) . Authors owe to thanks to HUT Culture Collection in supply of bacteria culture.en_US
dc.identifier.doi10.15832/ankutbd.866426
dc.identifier.endpage429en_US
dc.identifier.issn1300-7580
dc.identifier.issn2148-9297
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-85137914435en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage423en_US
dc.identifier.trdizinid1138129en_US
dc.identifier.urihttps://doi.org/10.15832/ankutbd.866426
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/1138129
dc.identifier.urihttps://hdl.handle.net/11616/92672
dc.identifier.volume28en_US
dc.identifier.wosWOS:000877379000008en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.publisherGalenos Publ Houseen_US
dc.relation.ispartofJournal of Agricultural Sciences-Tarim Bilimleri Dergisien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBioconversionen_US
dc.subjectBiphasic systemen_US
dc.subjectIsoeugenolen_US
dc.subjectNatural flavoren_US
dc.subjectVanillinen_US
dc.titleNatural Vanillin Production from Isoeugenol by Using Pseudomonas putida in Biphasic Bioconversion Mediumen_US
dc.typeArticleen_US

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