CA IX Inhibition by a Sulfonamide Compound: A Therapeutic Approach Against Breast Cancer
Küçük Resim Yok
Tarih
2026
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
John Wiley and Sons Inc
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Carbonic anhydrase IX (CA IX) is overexpressed in many solid tumors, contributing to cancer cell proliferation, survival, invasion, and metastasis. Sulfonamide-based compounds have emerged as potential anticancer agents by inhibiting this enzyme. In this study, we investigated the anticancer potential of a previously synthesized sulfonamide derivative, MMH-I, focusing on its CA IX-targeted activity and therapeutic efficacy in both in vitro and in vivo models of breast cancer. Cytotoxicity was assessed using MTT assays in 4T1 breast cancer cells, while apoptosis was evaluated by acridine orange/ethidium bromide staining and Annexin V detection. In vivo studies analyzed tumor tissues for CA IX expression, as well as Vimentin, E-Cadherin, and Caspase-3 levels. H&E staining and plasma metabolomic analysis were performed to assess tissue morphology and metabolic alterations. The compound significantly reduced tumor volume, induced apoptosis, and altered cancer-related gene expression and metabolic profiles. Overall, this study provides a detailed in vivo and metabolic evaluation of MMH-I in breast cancer, highlighting its potential as a CA IX–targeted therapeutic candidate and supporting further investigation of sulfonamide-based combination strategies against hypoxic tumors. © 2026 The Author(s). Chemistry & Biodiversity published by Wiley-VHCA AG.
Açıklama
Anahtar Kelimeler
4T1 Cancer Model, apoptosis, breast cancer, carbonic anhydrase IX, sulfonamide
Kaynak
Chemistry and Biodiversity
WoS Q Değeri
Scopus Q Değeri
Q2
Cilt
23
Sayı
3
