CA IX Inhibition by a Sulfonamide Compound: A Therapeutic Approach Against Breast Cancer

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dc.contributor.authorAkmese, Sükrü
dc.contributor.authorTemiz, Ebru
dc.contributor.authorGürel, Elif
dc.contributor.authorKoyuncu, Ismail
dc.contributor.authorDurgun, Mustafa
dc.contributor.authorGuldur, Muhammet Emin
dc.contributor.authorEgi, Kadir
dc.date.accessioned2026-04-04T13:18:51Z
dc.date.available2026-04-04T13:18:51Z
dc.date.issued2026
dc.departmentİnönü Üniversitesi
dc.description.abstractCarbonic anhydrase IX (CA IX) is overexpressed in many solid tumors, contributing to cancer cell proliferation, survival, invasion, and metastasis. Sulfonamide-based compounds have emerged as potential anticancer agents by inhibiting this enzyme. In this study, we investigated the anticancer potential of a previously synthesized sulfonamide derivative, MMH-I, focusing on its CA IX-targeted activity and therapeutic efficacy in both in vitro and in vivo models of breast cancer. Cytotoxicity was assessed using MTT assays in 4T1 breast cancer cells, while apoptosis was evaluated by acridine orange/ethidium bromide staining and Annexin V detection. In vivo studies analyzed tumor tissues for CA IX expression, as well as Vimentin, E-Cadherin, and Caspase-3 levels. H&E staining and plasma metabolomic analysis were performed to assess tissue morphology and metabolic alterations. The compound significantly reduced tumor volume, induced apoptosis, and altered cancer-related gene expression and metabolic profiles. Overall, this study provides a detailed in vivo and metabolic evaluation of MMH-I in breast cancer, highlighting its potential as a CA IX–targeted therapeutic candidate and supporting further investigation of sulfonamide-based combination strategies against hypoxic tumors. © 2026 The Author(s). Chemistry & Biodiversity published by Wiley-VHCA AG.
dc.description.sponsorshipHarran Üniversitesi, (21010); Harran Üniversitesi
dc.identifier.doi10.1002/cbdv.202501618
dc.identifier.issn1612-1872
dc.identifier.issue3
dc.identifier.scopus2-s2.0-105032663430
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1002/cbdv.202501618
dc.identifier.urihttps://hdl.handle.net/11616/107926
dc.identifier.volume23
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherJohn Wiley and Sons Inc
dc.relation.ispartofChemistry and Biodiversity
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_Scopus_20250329
dc.subject4T1 Cancer Model
dc.subjectapoptosis
dc.subjectbreast cancer
dc.subjectcarbonic anhydrase IX
dc.subjectsulfonamide
dc.titleCA IX Inhibition by a Sulfonamide Compound: A Therapeutic Approach Against Breast Cancer
dc.typeArticle

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