Evaluation of in vitro and in vivo toxic effects of newly synthesized benzimidazole-based organophosphorus compounds
Küçük Resim Yok
Tarih
2013
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Academic Press Inc Elsevier Science
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
This paper reports the toxic properties of eight newly synthesized benzimidazole-based organophosphorus (OP) compounds in Xeno pus laevis in both in vivo and in vitro conditions. For both experiments, a commercial solution of azinphos methyl (AzM, Gusathion M WP25) was used as a reference compound. The 24-h median lethal concentrations (LC50) of all tested compounds were determined for 46th stage tadpoles in the range of 9.54-140.0 mu M. For evaluation of the lethality of the compounds, the activity of the enzyme biomarkers acetylcholinesterase (AChE), carboxylesterase, glutathione S-transferase (GST), glutathione peroxidase, glutathione reductase, lactate dehydrogenase, aspartate aminotransferase, and alanine aminotransferase were determined in vivo in X. laevis tadpoles exposed to three concentrations (LC50, LC50/2, and LC50/4) of tested compounds. All exposure concentrations of AzM and seven of eight tested compounds caused CaE inhibition in in vivo conditions. Furthermore, the AChE inhibition capacity of tested compounds in commercial electric eel AChE and in X. laevis homogenates and also CaE inhibition capacity in only X. laevis homogenates were assayed for a 30-min in vitro exposure period. Eight OP compounds did not inhibit AChE activity more than 23 percent, but AzM exposure inhibited AChE activity by 26 percent for X. laevis homogenates and 97 percent for electric fish AChE in in vitro conditions. Also. CaE inhibition levels in X. laevis tadpole homogenates were 46 percent for AzM and between 8 percent and 33 percent for other compounds in in vitro conditions. (C) 2012 Elsevier Inc. All rights reserved.
Açıklama
Anahtar Kelimeler
Organophosphorus compounds, Acetylcholinesterase, Electric eel, Xenopus laevis, Biomarker
Kaynak
Ecotoxicology and Environmental Safety
WoS Q Değeri
Q2
Scopus Q Değeri
Q1
Cilt
87
