Antiinflammatory effects of cucurbitacins and sorafenib in Hepg2 cells by modulating the IκB/NF-κB/COX-2 pathway through Akt signaling
Küçük Resim Yok
Tarih
2025
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Tubitak Scientific & Technological Research Council Turkey
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Aim: Cucurbitacins possess antitumor, antiproliferative, and antiinflammatory properties. This study aims to examine the antiinflammatory effects of CuD, CuI, and CuE on NF-kappa B, iNOS, COX-2, and Akt and compare their cytotoxic and antiinflammatory effects on HepG2 cells with sorafenib, the primary chemotherapeutic agent used in HCC treatment. Methods: Cytotoxic effects of cucurbitacins and sorafenib on HepG2 cells were evaluated using MTT and LDH assays, along with Annexin V, MMP, and comet assays. Levels of proteins related to the Akt/NF-kappa B pathway and COX-2, iNOS, and NO were also measured. Results: Our study showed that CuD, CuI, CuE, and sorafenib have antiproliferative and cytotoxic effects on HepG2 cells. Cucurbitacins induced apoptosis at lower concentrations than sorafenib and, like sorafenib, reduced p-Akt, p-I kappa B alpha protein levels, and nuclear translocation of NF-kappa B in a dose-dependent manner. Moreover, all compounds significantly decreased COX-2, iNOS, and NO levels, Conclusion: These results indicate that cucurbitacins exert antiinflammatory effects on HepG2 cells by modulating the PI3K/Akt/NF kappa B signaling pathway, thereby reducing COX-2, iNOS, and NO levels. These effects are similar to those of sorafenib.
Açıklama
Anahtar Kelimeler
Cucurbitacin, sorafenib, HepG2, inflammation, NF kappa B, COX-2
Kaynak
Turkish Journal of Biology
WoS Q Değeri
Q3
Scopus Q Değeri
Q2
Cilt
49
Sayı
3
