Antiinflammatory effects of cucurbitacins and sorafenib in Hepg2 cells by modulating the IκB/NF-κB/COX-2 pathway through Akt signaling

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dc.contributor.authorUremis, Muhammed Mehd
dc.contributor.authorTurkoz, Yusuf
dc.contributor.authorUremis, Nuray
dc.date.accessioned2026-04-04T13:30:42Z
dc.date.available2026-04-04T13:30:42Z
dc.date.issued2025
dc.departmentİnönü Üniversitesi
dc.description.abstractAim: Cucurbitacins possess antitumor, antiproliferative, and antiinflammatory properties. This study aims to examine the antiinflammatory effects of CuD, CuI, and CuE on NF-kappa B, iNOS, COX-2, and Akt and compare their cytotoxic and antiinflammatory effects on HepG2 cells with sorafenib, the primary chemotherapeutic agent used in HCC treatment. Methods: Cytotoxic effects of cucurbitacins and sorafenib on HepG2 cells were evaluated using MTT and LDH assays, along with Annexin V, MMP, and comet assays. Levels of proteins related to the Akt/NF-kappa B pathway and COX-2, iNOS, and NO were also measured. Results: Our study showed that CuD, CuI, CuE, and sorafenib have antiproliferative and cytotoxic effects on HepG2 cells. Cucurbitacins induced apoptosis at lower concentrations than sorafenib and, like sorafenib, reduced p-Akt, p-I kappa B alpha protein levels, and nuclear translocation of NF-kappa B in a dose-dependent manner. Moreover, all compounds significantly decreased COX-2, iNOS, and NO levels, Conclusion: These results indicate that cucurbitacins exert antiinflammatory effects on HepG2 cells by modulating the PI3K/Akt/NF kappa B signaling pathway, thereby reducing COX-2, iNOS, and NO levels. These effects are similar to those of sorafenib.
dc.identifier.doi10.55730/1300-0152.2747
dc.identifier.issn1300-0152
dc.identifier.issn1303-6092
dc.identifier.issue3
dc.identifier.pmid40678417
dc.identifier.scopus2-s2.0-105010383351
dc.identifier.scopusqualityQ2
dc.identifier.trdizinid1334366
dc.identifier.urihttps://doi.org/10.55730/1300-0152.2747
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1334366
dc.identifier.urihttps://hdl.handle.net/11616/108310
dc.identifier.volume49
dc.identifier.wosWOS:001523096900007
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherTubitak Scientific & Technological Research Council Turkey
dc.relation.ispartofTurkish Journal of Biology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WOS_20250329
dc.subjectCucurbitacin
dc.subjectsorafenib
dc.subjectHepG2
dc.subjectinflammation
dc.subjectNF kappa B
dc.subjectCOX-2
dc.titleAntiinflammatory effects of cucurbitacins and sorafenib in Hepg2 cells by modulating the IκB/NF-κB/COX-2 pathway through Akt signaling
dc.typeArticle

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