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Öğe The beneficial effects of 18 glycyrrhetinic acid following oxidative and neuronal damage in brain tissue caused by global cerebral ischemia reperfusion in a C57BL J6 mouse model(Neurol Sci, 2014) Öztanır, Mustafa Namık; Çiftçi, Osman; Çetin, Aslı; Durak, Mehmet Akif; Başak, Neşe; Akyuva, YenerThis study investigated the effects of 18bglycyrrhetinic acid (GA) on neuronal damage in brain tissue caused by global cerebral ischemia/reperfusion (I/R) in C57BL/J6 mice. All subjects (n = 40) were equally divided into four groups: (1) sham-operated (SH), (2) I/R, (3) GA, and (4) GA?I/R. The SH group was used as a control. In the I/R group, the bilateral carotid arteries were clipped for 15 min, and the mice were treated with the vehicle for 10 days. In the GA group, mice were given GA (100 mg/ kg) for 10 days following a median incision without carotid occlusion. In the GA?I/R group, the I/R model was applied to the mice exactly as in the I/R group, and they were then treated with the same dose of GA for 10 days. Cerebral I/R significantly induced oxidative stress via an increase in lipid peroxidaitons and a decrease in elements of the antioxidant defense systems. However, GA treatment was protective against the oxidative effects of I/R by inducing significant increases in antioxidant defense systems and a significant decrease of lipid peroxidations. Additionally, cerebral I/R increased the incidence of histopathological damage and apoptosis in brain tissue, but these neurodegenerative effects were eliminated by GA treatment. Therefore, the current study demonstrated that GA treatment effectively prevents oxidative and histological damage in the brain caused by global I/R. In this context, GA may be useful for the attenuation of the negative effects of global cerebral I/R and, in the future, it may be a viable and safe alternative treatment for ischemic stroke in humans.Öğe Beneficial effects of curcumin and capsaicin on cyclophosphamide-induced premature ovarian failure in a rat model(BMC, CAMPUS, 4 CRINAN ST, LONDON N1 9XW, ENGLAND, 2018) Melekoğlu, Rauf; Çiftçi, Osman; Çetin, Aslı; Başak, NeşeBackground: In recent years, cancer rates have been rising among reproductive-age women. Thus, chemotherapy exposure has become an important cause of premature ovarian failure (POF). There has been growing interest regarding the preservation and restoration of ovarian function before and after oncological treatment because of the reproductive risk of chemotherapeutics and improved long-term survival of cancer patients. In this study, we sought to analyze the effects of curcumin (CRC) and capsaicin (CPS) on cyclophosphamide-induced POF in a rat model. Methods: POF in rats was induced by intraperitoneal injection of 200 mg/kg cyclophosphamide on day 1 and then 8 mg/kg/day for the following 14 days. After 14 days of cyclophosphamide administration, rats were randomly divided into three groups as follows (n = 10/group): POF, POF + CRC (100 mg/kg/day), and POF + CPS (0.5 mg/kg/day) to determine the effects of CRC and CPS on the cyclophosphamide-induced POF rat model. Biochemical, hormonal, and histopathological evaluations were performed on blood and tissue samples 14 days after the CRC and CPS treatments. Results: Malonaldehyde levels were significantly reduced, and glutathione levels and superoxide dismutase activity were significantly increased, in ovarian tissues in the POF + CRC and POF + CPS groups compared with the POF group. In the POF group, we observed hemorrhage and prominent mononuclear cell infiltration beneath the germinative epithelium, vascular congestion in ovarian stroma, hemorrhage around the corpus luteum, and atresia in ovarian follicles. This histopathological damage was significantly improved by treatment with CRC and CPS. There was a significant reduction in serum follicle-stimulating hormone and luteinizing hormone levels in rats treated with CRC and CPS compared with the POF group. Moreover, the levels of estradiol and anti-mullerian hormone in rats treated with CRC and CPS were significantly increased compared with the control group. Conclusions: In conclusion, CRC and CPS treatment of rats with cyclophosphamide-induced POF had a beneficial effect on reducing ovarian damage by improving tissue oxidative stress marker levels, ovarian reserve marker levels, and histopathological parameters. The significant improvements in ovarian tissue histopathological damage and hormonal levels detected in this study indicate that treatment with CRC or CPS might be a conservative treatment approach for cyclophosphamide-induced POF.Öğe Beneficial effects of glucan against cisplatin side effects on the nervous system in rats 1(Acta cir brasl, 2016) Kaya, Kürşat; Çiftçi, Osman; Çetin, Aslı; Tecellioğlu, Mehmet; Başak, NeşePURPOSE: To investigate the protective effect of Bg on cisplatin (CP)-induced neurotoxicity in rats. METHODS: Twenty eight rats were randomly distributed into four groups. The first group was kept as a control. In the second group, CP was given at the single dose of 7 mg/kg intraperitoneally. In the third group, βg was orally administered at the dose of 50 mg/kg/day for 14 days. In the fourth group, CP and βg were given together at the same doses. RESULTS: CP treatment caused significant oxidative damage via induction of lipid peroxidation and reductions antioxidant defense system potency in the brain tissue. In addition, histopathological damage increased with CP treatment. On the other hand, βg treatment largely prevented oxidative and histopathological negative effects of CP. CONCLUSIONS: Cisplatin has severe neurotoxic effects in rats and βg supplementation has significant beneficial effects against CP toxicity depending on its antioxidant properties. Thus, it appears that βg might be useful against CP toxicity in patients with cancer in terms of nervous system.Öğe Beneficial effects of hesperidin following cis diamminedichloroplatinum induced damage in heart of rats(Niger J Clin Pract., 2016) Oğuztürk, Hakan; Çiftçi, Osman; Çetin, Aslı; Kaya, Kürşat; Dişli, Om; Turtay, Muhammet Gökhan; Gürbüz, Şükrü; Başak, NeşeBackground: Increased oxidative stress and histopathological damage have been implicated in the cardiotoxicity that limits the clinical therapy of cisplatin (CP) as an anti-cancer drug. Objectives: This study aimed to investigate the protective effect of hesperidin (HP) against CP-induced cardiotoxicity in rats. Materials and Methods: Rats were divided into four groups (n = 7/group), and the first group served as the control group. Animals in Group CP and Group CP + HP received a single dose of CP (CP - 7 mg/kg); animals in Group HP and Group CP + HP received 50 mg/kg/day HP with gavage for 14 days. At the end of day 14, cardiac tissue samples were histologically and biochemically examined. Results: In this experimental study, thiobarbituric acid reactive substances levels in the cardiac tissue were significantly higher in the CP group, whereas glutathione (GSH), superoxide dismutase (SOD), and CAT levels were significantly lower in this group. On the other hand, GSH and SOD levels in the CP + HP group were similar to the control group. There was no significant difference in cardiac CAT levels between Group CP and Group CP + HP. Conclusion: Hesperetin treatment leads to a decrease in oxidative stress, and associated histological damage. The findings of the current study suggest that HP has a protective effect against CP‑induced cardiotoxicity.Öğe The beneficial effects of Montelukast against 2 3 7 8 tetrachlorodibenzo p dioxin toxicity in female reproductive system in rats(Acta Cir Bras., 2016) Melekoğlu, Rauf; Çiftçi, Osman; Çetin, Aslı; Başak, Neşe; Çelik, EbruPURPOSE: To determine the toxic effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on reproductive system and the beneficial effects of Montelukast (ML) with histological and biochemical analysis. METHODS: Rats were randomly divided into four equal groups (control, TCDD, ML and TCDD+ML). Tissue samples were collected on day 60 and oxidative status and histological alterations were analyzed. RESULTS: The results showed a significant increase in oxidative and histological damage on uterine and ovarian tissues. Otherwise, the oxidative and histological damages caused by TCDD were prevented with ML treatment. CONCLUSION: The toxic effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin on female reproductive system were reversed with Montelukast treatment. Therefore, we claimed that ML treatment might be useful for TCDD toxicity.Öğe Hesperidin a citrus flavonoid has the ameliorative effects against experimental autoimmune encephalomyelitis EAE in a C57BL J6 mouse model(Neurochemical Research, 2015) Çiftçi, Osman; Özcan, Abdulcemal; Kamışlı, Özden; Çetin, Aslı; Başak, Neşe; Bilal, AytaçThe aim of this study was determined the effects of Hesperidin (HP) on neuronal damage in brain tissue caused by Experimental allergic encephalomyelitis (EAE), an established model of multiple sclerosis in C57BL/J6 mice. To explore 40 mice were equally divided into four groups: (1) Control, (2) EAE, (3) HP, and (4) HP ? EAE. 14 days after induction of EAE with MOG35- 55 and pertussis toxin, the mice treated with HP at the doses of 50 mg/kg/day for 7 days subcutaneously. To our results HP treatment prevents the oxidative stress caused by EAE via a decrease in lipid peroxidations and increase in elements of the antioxidant defense systems in brain tissue. Also, EAE elevate the IL-17, express the pro-in- flammatory cytokines, and caspase-3-like immunreactivity, show apoptosis, staining in EAE mice brain and increased the incidence of histopathological damage. However, immonohistochemical and histological changes were reversed with HP. Moreover, elevated TNF-a and IL-1b levels, a result of EAE, were decreased in serum and neurological deficits as clinical signs were reversed with HP treatment in EAE mice, given HP. In conclusion, HP treatment effectively prevents oxidative, immunological and histological damage in the brain caused by EAE. It was thought that the beneficial effects of HP are likely a result of its strong antioxidant and anti-inflammatory properties.Öğe Hesperidin a citrus flavonoid has the ameliorative effects against experimental autoimmune encephalomyelitis EAE in a C57BL J6 mouse model(Neurochem Res, 2015) Çiftçi, Osman; Özcan, Cemal; Kamışlı, Özden; Çetin, Aslı; Başak, Neşe; Aytaç, BilalThe aim of this study was determined the effects of Hesperidin (HP) on neuronal damage in brain tissue caused by Experimental allergic encephalomyelitis (EAE), an established model of multiple sclerosis in C57BL/J6 mice. To explore 40 mice were equally divided into four groups: (1) Control, (2) EAE, (3) HP, and (4) HP ? EAE. 14 days after induction of EAE with MOG35- 55 and pertussis toxin, the mice treated with HP at the doses of 50 mg/kg/day for 7 days subcutaneously. To our results HP treatment prevents the oxidative stress caused by EAE via a decrease in lipid peroxidations and increase in elements of the antioxidant defense systems in brain tissue. Also, EAE elevate the IL-17, express the pro-in- flammatory cytokines, and caspase-3-like immunreactivity, show apoptosis, staining in EAE mice brain and increased the incidence of histopathological damage. However, immonohistochemical and histological changes were reversed with HP. Moreover, elevated TNF-a and IL-1b levels, a result of EAE, were decreased in serum and neurological deficits as clinical signs were reversed with HP treatment in EAE mice, given HP. In conclusion, HP treatment effectively prevents oxidative, immunological and histological damage in the brain caused by EAE. It was thought that the beneficial effects of HP are likely a result of its strong antioxidant and anti-inflammatory properties.Öğe Hesperidin protects testicular and spermatological damages induced by cisplatin in rats(Andrologia, 2015) Kaya, Kürşat; Çiftçi, Osman; Çetin, Aslı; Doğan, Halef; Başak, NeşeThe clinic usage of cisplatin, an anticancer drug, is limited due to it has many side effects in many systems and organs. In this context, it was aimed to investigate the protective effect of hesperidin, a citrus flavonoid, on testicular and spermatological damages induced by cisplatin in rats. The rats were randomly divided into four groups. The first group was kept as a control. In the second groups, cisplatin was given at the single dose of 7 mg kg(-1) intraperitoneally. In the third group, hesperidin was orally administered at the dose of 50 mg/kg day(-1) for 14 days. In the fourth group, cisplatin and hesperidin were given together at the same doses. Cisplatin treatment caused significant reductions enzymatic (SOD, CAT and GPx) and nonenzymatic (GSH) antioxidants and significant induction level of TBARS. In addition, cisplatin treatment caused decreased sperm motility, epididymal sperm concentration, increased abnormal sperm rate and histopathological damage. In contrast, hesperidin treatment significantly attenuated the harmful effects. In conclusion, this study clearly demonstrated that hesperidin has protective effects on cisplatin-induced reproductive system toxicity depending on its antioxidant properties. Thus, it is thought that hesperidin may be useful against cisplatin toxicity in patients with cancer in terms of reproductive system.Öğe Mechanical properties and cytotoxicity of hydroxyapatite based bone grafts applied mechanics and materials(Applied Mechanics and Materials, 2013) Demirel, Mehtap; Aksakal, Bünyamin; Başak, NeşeHydroxyapatite (HA) based bone grafts were synthesized by sol-gel method at various pH values 5, 7, 9 and 12, respectively. In order to ascertain the influence of the obtained bone grafts on the morphology, sinterability and mechanical properties, each composition was sintered at 11500C, and quantitative phase analysis was characterized by XRD and FTIR. The microstructures were studied via SEM-EDX and the validation of mechanical properties was evaluated by compression and Vickers hardness tests. The density of HA synthesis samples with different pH values before and after sintering were measured. Besides, the cytotoxicity analysis were conducted for all grafts having various pH values. The experimental results revealed that the density and mechanical strenght of HA based bone grafts were increased with increasing pH value. The highest hardness and strength were obtained for the samples having pH=12. Furthermore, the samples with pH=12 exhibited the best densification and properties when compared to the samples at pH of 5, 7 and 9. The cytotoxicity tests also indicated that the pH=7-12 value showed no toxicity to osteoblast cells at 0,1 and 0,3 μM concentration, whereas HA synthesis sample at pH=5 value with 0,3 μM concentration induced significantly and reduced cell viability.Öğe A novel schiff base derivative for effective treatment of azoxymethane induced colon cancer(IJPSR, 2014) Doğan, Ayşegül; Başak, Neşe; Demirci, Selami; Telci, Dilek; Dede, Bülent; Tuzcu, Mehmet; Özercan, İbrahim Halil; Şahin, Kazım; Şahin, FikrettinThe field of cancer research has been emerged in recent years for the development of specific drugs to cancer treatment. New agents with the ability to provide efficient treatment by reducing side effects has led to new opportunities for improving agents for cytotoxic therapies. While there are several drugs for colon cancer treatment, researchers are trying to evaluate new agents or combinations of existing ones which can be used efficiently. Schiff bases with a wide range of variety and biological properties including anticancer activity might be used for colon cancer treatment. In the current study, a novel schiff base derivative synthesized by our group was tested in vivo for colon cancer. In a model of azoxymethane (AOM) induced colorectal cancer, chemopreventive properties of schiff base was also analyzed in rats. While AOM induced de novo crypt formation, adenocarcinoma and dysplasia development, schiff base application reduced the number of aberrant crypt foci (ACF), dysplasia or adenocarcinoma. Analysis of the intestinal mucosa showed that peritoneal administration of SB complex not only decreased the protein expression of COX-2, Bcl-2 and NF-κB but also enhanced the Bax expression suggesting the apoptotic and anti-proliferative effects for this compound. Our findings showed that SB complex might be used for the colorectal cancer treatment. Further studies are highly warranted to obtain additional insights and identify mode of action for the schiff base.Öğe Oral administration of hesperidin a citrus flavonone in rats counteracts the oxidative stress the inflammatory cytokine production and the hepatotoxicity induced by the ingestion of 2 3 7 8 tetrachlorodibenzo p dioxin TCDD(Eur.Cytokine Netw, 2013) Bentli, Recep; Çiftçi, Osman; Çetin, Aslı; Ünlü, Merve; Başak, Neşe; Çay, MahmutThe objective of the current study was to investigate the protective effects of hesperidin against oxidative stress, altered cytokines levels and histological changes in rats induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Rats were divided randomly into four equal groups (Control, TCDD, hesperidin and TCDD+hesperidin). TCDD and hesperidin were given by gavage, dissolved in corn oil at doses of 2 /kg/week and 50 mg/kg/day respectively. The blood and tissue samples were taken from all rats on the 60th day, to be analyzed for the determination of oxidative stress, histological changes and cytokine levels. The results indicated that hesperidin prevented oxidative damage caused by TCDD via decrease lipid peroxidation and increased antioxidant defense systems. It also reversed the histological damage induced by TCDD. Although, TCDD led to a significant increase in TNF- and IL-1 levels, hesperidin treatment was able to normalize these values in rats. In conclusion, it was shown that TCDD caused adverse effects as regards cytokine levels, histological alterations and oxidative stress in rats. However, hesperidin treatment mitigated these toxic effects. These results suggest that hesperidin could play a protective role against TCDD toxicity.Öğe The protective cardiac effects of myrcene after global cerebral ıschemia reperfusion in C57BL J6 mouse(Acta cir bras, 2016) Baykalır, Burcu Gül; Çiftçi, Osman; Çetin, Aslı; Öztanır, Mustafa Namık; Başak, NeşeABSTRACT PURPOSE: To investigate the protective effect of β-myrcene (MYR) on oxidative and histological damage in mice heart tissue caused global cerebral ischemia/reperfusion (IR) in C57BL/J6 mice. METHODS: Animals(n=40) were randomly divided into four groups: (1)control, (2)IR, (3)MYR and (4)MYR+IR. The control group was received 0.1% carboxymethyl cellulose as a vehicle following a medial incision without carotid occlusion. In the IR group, the bilateral carotid arteries were clipped for 15min, and treated with the vehicle intraperitoneally(ip) for 10 days. MYR (200mg/kg) was received dissolved in 0.1%CMC for 10 days. In the MYR+IR group, the IR model was applied exactly as in the IR group, and then they were treated with MYR 10 days. RESULTS: The cerebral IR caused oxidative damage (increase TBARS, decrease antioxidant parameters). Treatment of MYR was increased in GSH,GPx,CAT,SOD activity while TBARS level was decreased. In addition, degenerative changes in I/R group heart tissue were ameliorated by MYR administration. CONCLUSİON: The administration of β-myrcene protects oxidative and histological damage in the heart tissue after global ischemiareperfusion and may be useful safe alternative treatment for cardiac tissue after ischemic stroke.Öğe Protective role of Diospyros lotus on cisplatin induced changes in sperm characteristics testicular damage and oxidative stress in rats(Andrologia, 2016) Saral, Sinan; Özçelik, E; Saral, O.; Çetin, Aslı; Başak, Neşe; Çiftçi, Osman; Aydın, MuhteremThe aim of this study was to investigate the protective effect of Diospyros lotus (DL) on cisplatin (CP)-induced testicular damage in male rats. Twenty-eight male rats were randomly divided into four groups: group 1 – control, given isotonic saline solution; group 2 – CP 7 mg kg 1 given intraperitoneally as single dose; group 3 – DL 1000 mg kg 1 per day given orally for 10 days; group 4 – CP and DL given together at the same doses. CP caused a significant increase in thiobarbituric acid-reactive substances (TBARS) level and a significant decrease in superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) and glutathione (GSH) levels in rats testis tissues compared to the control group. CP caused a significant increase in lipid peroxidation in testis tissues compared to the control group, whereas DL led to a significant increase in SOD and GSH levels. However, there were no statistically significant changes in GPx and CAT levels. In addition, serum testosterone levels, sperm concentration and sperm motility were significantly decreased, but abnormal sperm rate and histological changes were increased with CP. However, these effects of CP on sperm parameters, histological changes and the tissue weights were eliminated by DL treatment. In conclusion, our study showed that the reproductive toxicity caused by CP may be prevented by DL treatment.Öğe A Schiff base derivative for effective treatment of diethylnitrosamine induced liver cancer in vivo(Anticancer Drugs, 2015) Demirci, Selami; Doğan, Ayşegül; Başak, Neşe; Dede, Bülent; Telci, Dilek; Orhan, Cemal; Tuzcu, Mehmet; Şahin, Kazım; Şahin, Nurhan; Özercan, İbrahim; Şahin, FikrettinHepatocellular carcinoma is one of the most prevalent cancers, with a high morbidity rate, even in developed countries. In the present study, the curative effect of the Schiff base (SB) heterodinuclear copper(II)Mn(II) complex on diethylnitrosamine (DEN)-induced liver carcinoma was investigated. Hepatocarcinoma was initiated by an injection of DEN and promoted by phenobarbital (0.05%) in the diet. In addition, the potential nephrotoxicity of SB was evaluated in a cisplatin-induced nephrotoxicity model. Rats were administered the SB complex (1 and 2 mg/kg body weight/day) for 24 weeks, and cancer progression was investigated by macroscopic, histopathological, and western blot examinations. The administration of SB decreased the incidence and the number of hepatic nodules in a dose-dependent manner by regulating inflammation response and the apoptotic pathway. Western blot analyses from the livers of rats treated with SB after DEN induction showed significantly enhanced Bax and caspase-3 levels, with a marked decrease in the levels of Bcl-2, NF-κB p65 and cyclooxygenase (COX)-2. Results from the nephrotoxicity study showed that, whereas cisplatin increased serum urea nitrogen and creatinine levels, no increase in serum biochemical parameters was detected in SB-treated animals. Moreover, protein levels of NF-E2-related factor-2 (Nrf2) and heme oxygenase-1 were lower, whereas nuclear factor-κB (NF-κB p65) and activator protein-1 levels were higher in the kidneys of cisplatin-treated animals compared with that of the SB groups. Therefore, the SB complex could be an alternative chemotherapeutic option for liver cancer treatment once its safety in clinical applications has been examined.Öğe Schiff base poloxamer P85 combination prevents prostate cancer progression in C57 Bl6 mice(Prostate, 2016) Doğan, Ayşegül; Demirci, Selami; Başak, Neşe; Çağlayan, Ahmet Burak; Aydın, Safa; Telci, Dilek; Kılıç, Ertuğrul; Şahin, Kazım; Orhan, Cemal; Tuzcu, Mehmet; Şahin, Fikrettin; Doğan Ekinci, Işın AsiyeBACKGROUND. Prostate cancer which is the second most common cause of death among men has a high incidence in recent years. Current therapeutic regimens should be improved to overcome drug resistance. At the metastatic stage, tumors become refractory to established chemotherapeutic treatments and cause serious problems at the clinics. Development of new drug molecules that are able to transport through the membrane easily and kill tumor cells rapidly is of great interest. METHOD. In the current study, a novel Heterodinuclear copper(II)Mn(II) Schiff base complex combined with P85 was used for prostate cancer treatment in vivo. Tramp-C1 cells injected animals were subjected to chemotherapeutic formulation treatment and results were analyzed by toxicology analysis, tumor volume measurements, and histopathological analysis. 0.5 mg/kg Schiff base was selected and combined with 0.05% P85 according to the toxicology analysis showing the enzyme levels, blood parameters, and multiple organ toxicity. RESULTS. Results demonstrated that Heterodinuclear copper(II)Mn(II) complex-P85 combination decreased tumor formation and tumor volume steadily over the course of experiments. CONCLUSIONS. Overall, Heterodinuclear copper(II)Mn(II) complex-P85 exerted remarkable anti-cancer activity in vivo in C57/B16 mice. Prostate 76:1454–1463, 2016. # 2016 Wiley Periodicals, Inc.Öğe Sensitization of cervical cancer cells to cisplatin by genistein the role of NF B and akt mTOR signaling pathways(Journal of Oncology Volume, 2012) Şahin, Kazım; Tuzcu, Mehmet; Başak, Neşe; Çağlayan, Ahmet Burak; Kılıç, Ertuğrul; Şahin, Fikrettin; Küçük, ÖmerCervical cancer is among the top causes of death from cancer in women. Cisplatin-based chemotherapy has been shown to improve survival; however, cisplatin treatment is associated with toxicity to healthy cells. Genistein has been used as an adjunct to chemotherapy to enhance the activity of chemotherapeutic agents without causing increased toxicity. The present study was designed to investigate the effect of genistein (25 μM) on antitumor activity of cisplatin (250 nM) on HeLa cervical cancer cells. We have examined the alterations in expression of NF-B, p-mTOR, p-p70S6K1, p-4E-BP1, and p-Akt protein levels in response to treatment. The combination of 25 μM genistein with 250 nM cisplatin resulted in significantly greater growth inhibition (). Genistein enhanced the antitumor activity of cisplatin and reduced the expression of NF-B, p-mTOR, p-p70S6K1, p-4E-BP1, and p-Akt. The results in the present study suggest that genistein could enhance the activity of cisplatin via inhibition of NF-κB and Akt/mTOR pathways. Genistein is a promising nontoxic nutritional agent that may enhance treatment outcome in cervical cancer patients when given concomitantly with cisplatin. Clinical trials of genistein and cisplatin combination are warranted to test this hypothesis.Öğe Yeni sentezlenen gümüş karben bileşiklerinin sitotoksik ve antimikrobiyal aktivitelerinin araştırılması(İnönü Üniversitesi, 2014) Başak, NeşeBu çalışmada, İnönü Üniversitesi Anorganik Kimya Araştırma laboratuvarında orjinal olarak sentezlenmiş 6 adet benzimidazol grubu gümüş karben komplekslerinin beyin kanseri hücre hattı (SHSY5Y) ve karaciğer kanseri hücre hattı (HEP3B) üzerinde sitotoksik etkisi MTS canlılık testiyle belirlenmiştir. Kontrol grubu olarak sağlıklı fibroblast hücreleri (HF) kullanılmıştır. Toksisite testlerinin sonucu 3 gün süreyle ve 24 saat aralıklarla ELISA cihazında okunmuş ve hücrelerin canlılık oranı spektrofotometrik olarak (490 nm) belirlenmiştir. Test edilen gümüş karben komplekslerinden 5 tanesinin beyin ve karaciğer kanserli hücre hatlarında doza bağımlı bir toksisiteye neden olduğu gözlenmiş ve bu hücre hatlarında 3 günlük inkübasyon sonucu %100'e varan hücre ölümleri saptanmıştır. Bununla birlikte her bir kompleks için minimal inhibitör konsantrasyonları (MİK) referans bakteri ve mantar suşlarına karşı denenmiş ve elde edilen sonuçlara göre antifungal etkileri antibakteriyel etkilerine göre daha yüksek bulunmuştur. Tez çalışmasının bulgularına göre, bu çalışmada kullanılan gümüş karben komplekslerinin kanserli hücre hatlarında, sağlıklı hücreye zarar vermeden etkin hücre ölümüne sebep olduğunu görülmektedir. Devam eden çalışmalar ile bu moleküllerin mevcut diğer kanser hücre hatları ve in-vivo modeller üzerindeki tedavi potansiyelleri araştırılacaktır.
